A Chrnb3-Cre BAC transgenic mouse line for manipulation of gene expression in retinal ganglion cells.

Autor: Drayson LE; Center for Neuroscience Research, Children's National Medical Center, Washington, DC., Triplett JW; Center for Neuroscience Research, Children's National Medical Center, Washington, DC.; Depts. of Pediatrics and Pharmacology & Physiology, The George Washington University School of Medicine and Health Sciences, Washington, DC.
Jazyk: angličtina
Zdroj: Genesis (New York, N.Y. : 2000) [Genesis] 2019 Sep; Vol. 57 (9), pp. e23305. Date of Electronic Publication: 2019 May 14.
DOI: 10.1002/dvg.23305
Abstrakt: The mechanisms by which retinal ganglion cells (RGCs) make specific connections during development is an intense area of research and have served as a model for understanding the general principles of circuit wiring. As such, genetic tools allowing for specific recombination in RGCs are critical to further our understanding of the cell-specific roles of different genes during these processes. However, many RGC-specific Cre lines have drawbacks, due to their broad expression in other cell types and/or retinorecipient regions or lack of expression in broad swaths of the retina. Here, we characterize a Cre BAC transgenic line driven by elements of the cholinergic receptor nicotinic beta 3 subunit (Chrnb3). We show that Cre expression is restricted to RGCs in the retina and sparsely expressed in the brain, importantly excluding retinorecipient regions. Furthermore, Chrnb3-Cre mice label a wide variety of RGCs distributed throughout the retina and Cre activity is detected embryonically, shortly following RGC differentiation. Finally, we find that Chrnb3-Cre-labeled RGCs innervate multiple retinorecipient areas that serve both image-forming and nonimage forming functions. Thus, this genetic tool will be of broad use to investigators studying the RGC-specific contributions of genes to visual circuit development.
(© 2019 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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