Flow Cytometry Characterization of Cerebrospinal Fluid Monocytes in Patients With Postoperative Cognitive Dysfunction: A Pilot Study.

Autor:	Berger M; From the Anesthesiology Department, Duke University Medical Center, Durham, North Carolina.; Center for the Study of Aging and Human Development, Duke University Medical Center, Durham, North Carolina.; Center for Cognitive Neuroscience, Duke Institute for Brain Sciences, Duke University, Durham, North Carolina., Murdoch DM; Department of Medicine, Duke University Medical Center, Durham, North Carolina., Staats JS; Surgical Oncology Research Facility, Surgery Department, Duke University Medical Center, Durham, North Carolina., Chan C; Surgical Oncology Research Facility, Surgery Department, Duke University Medical Center, Durham, North Carolina., Thomas JP; From the Anesthesiology Department, Duke University Medical Center, Durham, North Carolina.; Trinity College, Duke University, Durham, North Carolina., Garrigues GE; Department of Orthopedics, Duke University Medical Center, Durham, North Carolina., Browndyke JN; Center for Cognitive Neuroscience, Duke Institute for Brain Sciences, Duke University, Durham, North Carolina.; Department of Psychiatry and Behavioral Sciences, Duke University Medical Center, Durham, North Carolina., Cooter M; From the Anesthesiology Department, Duke University Medical Center, Durham, North Carolina., Quinones QJ; From the Anesthesiology Department, Duke University Medical Center, Durham, North Carolina., Mathew JP; From the Anesthesiology Department, Duke University Medical Center, Durham, North Carolina., Weinhold KJ; Surgical Oncology Research Facility, Surgery Department, Duke University Medical Center, Durham, North Carolina.
Jazyk:	angličtina
Zdroj:	Anesthesia and analgesia [Anesth Analg] 2019 Nov; Vol. 129 (5), pp. e150-e154.
DOI:	10.1213/ANE.0000000000004179
Abstrakt:	Animal models suggest postoperative cognitive dysfunction may be caused by brain monocyte influx. To study this in humans, we developed a flow cytometry panel to profile cerebrospinal fluid (CSF) samples collected before and after major noncardiac surgery in 5 patients ≥60 years of age who developed postoperative cognitive dysfunction and 5 matched controls who did not. We detected 12,654 ± 4895 cells/10 mL of CSF sample (mean ± SD). Patients who developed postoperative cognitive dysfunction showed an increased CSF monocyte/lymphocyte ratio and monocyte chemoattractant protein 1 receptor downregulation on CSF monocytes 24 hours after surgery. These pilot data demonstrate that CSF flow cytometry can be used to study mechanisms of postoperative neurocognitive dysfunction.
Databáze:	MEDLINE
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