Snf2 controls pulcherriminic acid biosynthesis and antifungal activity of the biocontrol yeast Metschnikowia pulcherrima.

Autor: Gore-Lloyd D; Department of Biology & Biochemistry, University of Bath, Bath, BA2 7AY, UK., Sumann I; Agroscope, Research Division Plant Protection, Müller-Thurgau-Strasse 29, 8820, Wädenswil, Switzerland., Brachmann AO; Institute of Microbiology, Eidgenössische Technische Hochschule (ETH) Zürich, 8093, Zürich, Switzerland., Schneeberger K; Competence Division Method Development and Analytics, Müller-Thurgau-Strasse 29, 8820, Wädenswil, Switzerland., Ortiz-Merino RA; Conway Institute, University College Dublin, Dublin 4, Ireland., Moreno-Beltrán M; Department of Biology & Biochemistry, University of Bath, Bath, BA2 7AY, UK., Schläfli M; Agroscope, Research Division Plant Protection, Müller-Thurgau-Strasse 29, 8820, Wädenswil, Switzerland., Kirner P; Agroscope, Research Division Plant Protection, Müller-Thurgau-Strasse 29, 8820, Wädenswil, Switzerland., Santos Kron A; Agroscope, Research Division Plant Protection, Müller-Thurgau-Strasse 29, 8820, Wädenswil, Switzerland., Rueda-Mejia MP; Agroscope, Research Division Plant Protection, Müller-Thurgau-Strasse 29, 8820, Wädenswil, Switzerland., Somerville V; Competence Division Method Development and Analytics, Müller-Thurgau-Strasse 29, 8820, Wädenswil, Switzerland., Wolfe KH; Conway Institute, University College Dublin, Dublin 4, Ireland., Piel J; Institute of Microbiology, Eidgenössische Technische Hochschule (ETH) Zürich, 8093, Zürich, Switzerland., Ahrens CH; Competence Division Method Development and Analytics, Müller-Thurgau-Strasse 29, 8820, Wädenswil, Switzerland.; SIB, Swiss Institute of Bioinformatics, Müller-Thurgau-Strasse 29, 8820, Wädenswil, Switzerland., Henk D; Department of Biology & Biochemistry, University of Bath, Bath, BA2 7AY, UK., Freimoser FM; Agroscope, Research Division Plant Protection, Müller-Thurgau-Strasse 29, 8820, Wädenswil, Switzerland.
Jazyk: angličtina
Zdroj: Molecular microbiology [Mol Microbiol] 2019 Jul; Vol. 112 (1), pp. 317-332. Date of Electronic Publication: 2019 May 20.
DOI: 10.1111/mmi.14272
Abstrakt: Metschnikowia pulcherrima synthesises the pigment pulcherrimin, from cyclodileucine (cyclo(Leu-Leu)) as a precursor, and exhibits strong antifungal activity against notorious plant pathogenic fungi. This yeast therefore has great potential for biocontrol applications against fungal diseases; particularly in the phyllosphere where this species is frequently found. To elucidate the molecular basis of the antifungal activity of M. pulcherrima, we compared a wild-type strain with a spontaneously occurring, pigmentless, weakly antagonistic mutant derivative. Whole genome sequencing of the wild-type and mutant strains identified a point mutation that creates a premature stop codon in the transcriptional regulator gene SNF2 in the mutant. Complementation of the mutant strain with the wild-type SNF2 gene restored pigmentation and recovered the strong antifungal activity. Mass spectrometry (UPLC HR HESI-MS) proved the presence of the pulcherrimin precursors cyclo(Leu-Leu) and pulcherriminic acid and identified new precursor and degradation products of pulcherriminic acid and/or pulcherrimin. All of these compounds were identified in the wild-type and complemented strain, but were undetectable in the pigmentless snf2 mutant strain. These results thus identify Snf2 as a regulator of antifungal activity and pulcherriminic acid biosynthesis in M. pulcherrima and provide a starting point for deciphering the molecular functions underlying the antagonistic activity of this yeast.
(© 2019 The Authors. Molecular Microbiology Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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