| Autor: |
Fokin Artem I; A.N. Belozersky Institute for Physico-Chemical Biology, M.V. Lomonosov Moscow State University, Vorobjevy Gory, 1 bld.73, Moscow, Russian Federation, 119991., Zhapparova Olga N; A.N. Belozersky Institute for Physico-Chemical Biology, M.V. Lomonosov Moscow State University, Vorobjevy Gory, 1 bld.73, Moscow, Russian Federation, 119991., Burakov Anton V; A.N. Belozersky Institute for Physico-Chemical Biology, M.V. Lomonosov Moscow State University, Vorobjevy Gory, 1 bld.73, Moscow, Russian Federation, 119991., Nadezhdina Elena S; Department of Cell Biology of Institute of Protein Research, Russian Academy of Science, Vavilova ul., 34, Moscow, Russian Federation, 117334. nadezhdina@vega.protres.ru. |
| Abstrakt: |
In animal cells, the centrosome nucleates and anchors microtubules (MT), forming their radial array. During interphase centrosome-derived MT, aster can either team up with other MT network or function in an autonomous manner. What is the function of the centrosome-derived MT aster? We suggested that it might play an important role in the formation of the primary cilium, the organelle obligatorily associated with the centrosome. PCM-1 (PeriCentriolar Matrix 1) protein, which participates in the organization of the primary cilium, is a part of pericentiolar satellites. They are transported to the centrosome along MTs by the motor protein dynein in a complex with its cofactor dynactin. Previously, we showed that SLK/LOSK phosphorylated the p150 Glued subunit of dynactin, thus promoting its centrosomal targeting followed by its participation in the retention of microtubules. Here, we found that under the repression of SLK/LOSK activity, the PCM-1 protein lost its pericentrosomal localization and was being dispersed throughout the cytoplasm. Despite that the alanine and glutamine mutants of p150 Glued had opposite effects on PCM-1 localization, they associated with PCM-1 to the same extent. The occurrence of primary cilia also significantly decreased when SLK/LOSK was repressed. These defects also correlated with a disturbance of the long-range transport in cells, whereas dynein-depending motility was intact. Treatment with the GSK-3β kinase inhibitor also resulted in the loss of the centrosome-derived MT aster, dispersion of PCM-1 over the cytoplasm, and reduction of primary cilia occurrence. Thus, kinases involved in the centrosome-derived MT aster regulation can indirectly control the formation of primary cilia in cells. |
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