IncC bla KPC-2 -positive plasmid characterised from ST648 Escherichia coli.

Autor: Papagiannitsis CC; Department of Microbiology, University Hospital of Larissa, Larissa, Greece., Bitar I; Biomedical Center, Faculty of Medicine and University Hospital in Plzen, Charles University in Prague, Plzen, Czech Republic., Malli E; Department of Microbiology, University Hospital of Larissa, Larissa, Greece., Tsilipounidaki K; Department of Microbiology, University Hospital of Larissa, Larissa, Greece., Hrabak J; Biomedical Center, Faculty of Medicine and University Hospital in Plzen, Charles University in Prague, Plzen, Czech Republic., Petinaki E; Department of Microbiology, University Hospital of Larissa, Larissa, Greece. Electronic address: petinaki@med.uth.gr.
Jazyk: angličtina
Zdroj: Journal of global antimicrobial resistance [J Glob Antimicrob Resist] 2019 Dec; Vol. 19, pp. 73-77. Date of Electronic Publication: 2019 May 08.
DOI: 10.1016/j.jgar.2019.05.001
Abstrakt: Objectives: This study describes the characterisation of type 2 IncC plasmids pC-Ec20-KPC and pC-Ec2-KPC, carrying thebla KPC-2 gene, from two multiresistant Escherichia coli recovered in University Hospital of Larissa (Greece) in 2018.
Methods: E. coli strains Ec-2Lar and Ec-20Lar were recovered from rectal swabs of two patients during monthly surveillance cultures. Transfer experiments by conjugation were carried out using rifampicin-resistant E. coli A15 laboratory strain as recipient. bla KPC -carrying plasmids were characterised by S1 profiling. Isolates were typed by MLST. Whole-genome sequencing was performed using the Sequel platform.
Results: Both E. coli isolates, belonging to ST648, transferred bla KPC-2 to E. coli A15 by conjugation. Plasmid analysis revealed that the transconjugants harboured bla KPC -positive plasmids of different sizes. Analysis of plasmid sequences showed that in both isolates the bla KPC-2 gene was carried on a type 2 IncC plasmid (pC-Ec20-KPC and pC-Ec2-KPC, respectively). Both plasmids carried the ARI-B resistance island consisting of several resistance genes, intact and truncated copies of several mobile elements, and a 25 571-bp segment harbouring coding sequences for an iron transporter. The bla KPC-2 gene was part of transposon Tn4401a, which was bounded by 5-bp direct repeats (TCCTT) suggesting its transposition into the IncC plasmids.
Conclusion: To our knowledge, this is the first report on complete nucleotide sequences of type 2 IncC plasmids. These findings, which hypothesise the acquisition of KPC-2-encoding transposon Tn4401a by an IncC replicon, indicate the ongoing need for molecular surveillance studies of multidrug-resistant pathogens. In addition, they underline the increasing clinical importance of the IncC plasmid family.
(Copyright © 2019. Published by Elsevier Ltd.)
Databáze: MEDLINE
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