Visualization of Mass Cytometry Signal Background to Enable Optimal Core Panel Customization and Signal Threshold Gating.

Autor:	Au-Yeung A; OMNI Biomarkers, Development Sciences, Genentech, South San Francisco, CA, USA., Takahashi C; OMNI Biomarkers, Development Sciences, Genentech, South San Francisco, CA, USA., Mathews WR; OMNI Biomarkers, Development Sciences, Genentech, South San Francisco, CA, USA., O'Gorman WE; OMNI Biomarkers, Development Sciences, Genentech, South San Francisco, CA, USA. ogormanw@gene.com.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2019; Vol. 1989, pp. 35-45.
DOI:	10.1007/978-1-4939-9454-0_3
Abstrakt:	Signal interference or overlap in mass cytometry is minimal compared to flow cytometry but must still be considered for optimal panel design and assay sensitivity. Here we describe a procedure for evaluating signal interference dynamics in the context of a 25-parameter core immunophenotyping panel. Specifically, a mass-minus-many (MMM) approach was used to assess background signals in "empty" or "blank" channels intended for further customization. Through this approach cell type-specific variability in signal background is revealed. Further panel customization can thus be performed with an understanding of cell type and channel-specific background levels to enable rational panel design and the objective delineation of gating thresholds during analysis.
Databáze:	MEDLINE
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