Human umbilical cord blood cells restore vascular integrity in injured rat brain and modulate inflammation in vitro .

Autor: Srivastava AK; Department of Pediatric Surgery, McGovern Medical School, University of Texas Health Sciences Center at Houston, Houston, TX, 77030, USA., Prabhakara KS; Department of Pediatric Surgery, McGovern Medical School, University of Texas Health Sciences Center at Houston, Houston, TX, 77030, USA., Kota DJ; Emory Personalized Immunotherapy Core Labs, Emory University, School of Medicine, Atlanta, GA 30322, USA., Bedi SS; Department of Pediatric Surgery, McGovern Medical School, University of Texas Health Sciences Center at Houston, Houston, TX, 77030, USA., Triolo F; Department of Pediatric Surgery, McGovern Medical School, University of Texas Health Sciences Center at Houston, Houston, TX, 77030, USA., Brown KS; Cbr Systems, Inc., South San Francisco, CA, 94080, USA., Skiles ML; Cbr Systems, Inc., South San Francisco, CA, 94080, USA., Brown HL; Cbr Systems, Inc., South San Francisco, CA, 94080, USA., Cox CS Jr; Department of Pediatric Surgery, McGovern Medical School, University of Texas Health Sciences Center at Houston, Houston, TX, 77030, USA., Olson SD; Department of Pediatric Surgery, McGovern Medical School, University of Texas Health Sciences Center at Houston, Houston, TX, 77030, USA.
Jazyk: angličtina
Zdroj: Regenerative medicine [Regen Med] 2019 May; Vol. 14 (4), pp. 295-307. Date of Electronic Publication: 2019 May 10.
DOI: 10.2217/rme-2018-0106
Abstrakt: Aim: Traumatic brain injury is a complex condition consisting of a mechanical injury with neurovascular disruption and inflammation with limited clinical interventions available. A growing number of studies report systemic delivery of human umbilical cord blood (HUCB) as a therapy for neural injuries. Materials & methods: HUCB cells from five donors were tested to improve blood-brain barrier integrity in a traumatic brain injury rat model at a dose of 2.5 × 10 7 cells/kg at 24 or 72 h postinjury and for immunomodulatory activity in vitro . Results & Conclusion: We observed that cells delivered 72 h postinjury significantly restored blood-brain barrier integrity. HUCB cells reduced the amount of TNF-α and IFN-γ released by activated primary rat splenocytes, which correlated with the expression of COX2 and IDO1.
Databáze: MEDLINE