Infection and nuclear interaction in mammalian cells by 'Candidatus Berkiella cookevillensis', a novel bacterium isolated from amoebae.

Autor: Chamberlain NB; Molecular Biosciences Program, Middle Tennessee State University, 1301 E. Main St, Murfreesboro, TN, 37130, USA.; Department of Biology, Middle Tennessee State University, 1301 E. Main St, Murfreesboro, TN, 37130, USA., Mehari YT; Molecular Biosciences Program, Middle Tennessee State University, 1301 E. Main St, Murfreesboro, TN, 37130, USA.; Department of Biology, Middle Tennessee State University, 1301 E. Main St, Murfreesboro, TN, 37130, USA., Hayes BJ; Department of Biology, Middle Tennessee State University, 1301 E. Main St, Murfreesboro, TN, 37130, USA., Roden CM; Department of Biology, Middle Tennessee State University, 1301 E. Main St, Murfreesboro, TN, 37130, USA., Kidane DT; Molecular Biosciences Program, Middle Tennessee State University, 1301 E. Main St, Murfreesboro, TN, 37130, USA.; Department of Biology, Middle Tennessee State University, 1301 E. Main St, Murfreesboro, TN, 37130, USA., Swehla AJ; Department of Biology, Middle Tennessee State University, 1301 E. Main St, Murfreesboro, TN, 37130, USA., Lorenzana-DeWitt MA; Department of Biology, Middle Tennessee State University, 1301 E. Main St, Murfreesboro, TN, 37130, USA., Farone AL; Molecular Biosciences Program, Middle Tennessee State University, 1301 E. Main St, Murfreesboro, TN, 37130, USA.; Department of Biology, Middle Tennessee State University, 1301 E. Main St, Murfreesboro, TN, 37130, USA., Gunderson JH; Department of Biology, Tennessee Technological University, 1 William L Jones Dr, Cookeville, TN, 38505, USA., Berk SG; Department of Biology, Middle Tennessee State University, 1301 E. Main St, Murfreesboro, TN, 37130, USA., Farone MB; Molecular Biosciences Program, Middle Tennessee State University, 1301 E. Main St, Murfreesboro, TN, 37130, USA. mary.farone@mtsu.edu.; Department of Biology, Middle Tennessee State University, 1301 E. Main St, Murfreesboro, TN, 37130, USA. mary.farone@mtsu.edu.
Jazyk: angličtina
Zdroj: BMC microbiology [BMC Microbiol] 2019 May 09; Vol. 19 (1), pp. 91. Date of Electronic Publication: 2019 May 09.
DOI: 10.1186/s12866-019-1457-z
Abstrakt: Background: 'Candidatus Berkiella cookevillensis' and 'Ca. Berkiella aquae' have previously been described as intranuclear bacteria of amoebae. Both bacteria were isolated from amoebae and were described as appearing within the nuclei of Acanthamoeba polyphaga and ultimately lysing their host cells within 4 days. Both bacteria are Gammaproteobacteria in the order Legionellales with the greatest similarity to Coxiella burnetii. Neither bacterium grows axenically in artificial culture media. In this study, we further characterized 'Ca. B. cookevillensis' by demonstrating association with nuclei of human phagocytic and nonphagocytic cell lines.
Results: Transmission electron microscopy (TEM) and confocal microscopy were used to confirm nuclear co-localization of 'Ca. B. cookevillensis' in the amoeba host A. polyphaga with 100% of cells having bacteria co-localized with host nuclei by 48 h. TEM and confocal microscopy demonstrated that the bacterium was also observed to be closely associated with nuclei of human U937 and THP-1 differentiated macrophage cell lines and nonphagocytic HeLa human epithelial-like cells. Immunofluorescent staining revealed that the bacteria-containing vacuole invaginates the nuclear membranes and appears to cross from the cytoplasm into the nucleus as an intact vacuole.
Conclusion: Results of this study indicate that a novel coccoid bacterium isolated from amoebae can infect human cell lines by associating with the host cell nuclei, either by crossing the nuclear membranes or by deeply invaginating the nuclear membranes. When associated with the nuclei, the bacteria appear to be bound within a vacuole and replicate to high numbers by 48 h. We believe this is the first report of such a process involving bacteria and human cell lines.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje