Mitochondrial genomes of Anopheles arabiensis , An. gambiae and An. coluzzii show no clear species division.

Autor:	Hanemaaijer MJ; Vector Genetics Laboratory, Department of Pathology, Microbiology and Immunology, University of California Davis , Davis, CA, 95616, USA., Houston PD; Vector Genetics Laboratory, Department of Pathology, Microbiology and Immunology, University of California Davis , Davis, CA, 95616, USA., Collier TC; Vector Genetics Laboratory, Department of Pathology, Microbiology and Immunology, University of California Davis , Davis, CA, 95616, USA., Norris LC; Vector Genetics Laboratory, Department of Pathology, Microbiology and Immunology, University of California Davis , Davis, CA, 95616, USA., Fofana A; Malaria Research and Training Center, University of Bamako, Bamako, E2528, Mali., Lanzaro GC; Vector Genetics Laboratory, Department of Pathology, Microbiology and Immunology, University of California Davis , Davis, CA, 95616, USA., Cornel AJ; Mosquito Control Research Laboratory, Kearney Agricultural Center, Department of Entomology and Nematology, University of California Davis, Davis, CA, 93648, USA., Lee Y; Vector Genetics Laboratory, Department of Pathology, Microbiology and Immunology, University of California Davis , Davis, CA, 95616, USA.
Jazyk:	angličtina
Zdroj:	F1000Research [F1000Res] 2018 Mar 21; Vol. 7, pp. 347. Date of Electronic Publication: 2018 Mar 21 (Print Publication: 2018).
DOI:	10.12688/f1000research.13807.1
Abstrakt:	Here we report the complete mitochondrial sequences of 70 individual field collected mosquito specimens from throughout Sub-Saharan Africa. We generated this dataset to identify species specific markers for the following Anopheles species and chromosomal forms: An. arabiensis , An. coluzzii (The Forest and Mopti chromosomal forms) and An. gambiae (The Bamako and Savannah chromosomal forms).  The raw Illumina sequencing reads were mapped to the NC_002084 reference mitogenome sequence. A total of 783 single nucleotide polymorphisms (SNPs) were detected on the mitochondrial genome, of which 460 are singletons (58.7%). None of these SNPs are suitable as molecular markers to distinguish among An. arabiensis , An. coluzzii and An. gambiae or any of the chromosomal forms. The lack of species or chromosomal form specific markers is also reflected in the constructed phylogenetic tree, which shows no clear division among the operational taxonomic units considered here. Competing Interests: No competing interests were disclosed.
Databáze:	MEDLINE
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