Printing Therapeutic Proteins in 3D using Nanoengineered Bioink to Control and Direct Cell Migration.

Autor: Peak CW; Biomedical Engineering, Dwight Look College of Engineering, Texas A&M University, College Station, TX, 77843, USA., Singh KA; Biomedical Engineering, Dwight Look College of Engineering, Texas A&M University, College Station, TX, 77843, USA., Adlouni M; Biomedical Engineering, Dwight Look College of Engineering, Texas A&M University, College Station, TX, 77843, USA., Chen J; Biomedical Engineering, Dwight Look College of Engineering, Texas A&M University, College Station, TX, 77843, USA., Gaharwar AK; Biomedical Engineering, Dwight Look College of Engineering, Texas A&M University, College Station, TX, 77843, USA.; Material Science and Engineering, Dwight Look College of Engineering, Texas A&M University, College Station, TX, 77843, USA.; Center for Remote Health Technologies and Systems, Texas A&M University, College Station, TX, 77843, USA.
Jazyk: angličtina
Zdroj: Advanced healthcare materials [Adv Healthc Mater] 2019 Jun; Vol. 8 (11), pp. e1801553. Date of Electronic Publication: 2019 May 08.
DOI: 10.1002/adhm.201801553
Abstrakt: A nanoengineered bioink loaded with therapeutic proteins is designed to direct cell function in a 3D printed construct. The bioink is developed from a hydrolytically degradable polymer and 2D synthetic nanoparticle. The synthesis of poly(ethylene glycol)-dithiothreitol (PEGDTT) via a Michael-like step growth polymerization results in acrylate terminated degradable macromer. The addition of 2D nanosilicates to PEGDTT results in formation of shear-thinning bioinks with high printability and structural fidelity. The mechanical properties, swelling kinetics, and degradation rate of 3D printed constructs can be modulated by changing the ratio of PEG:PEGDTT and nanosilicates concentration. Due to high surface area and charged characteristic of nanosilicates, protein therapeutics can be sequestered in 3D printing structure for prolong duration. Sustained release of pro-angiogenic therapeutics from 3D printed structure, promoted rapid migration of human endothelial umbilical vein cell. This approach to design biologically active inks to control and direct cell behavior can be used to engineer 3D complex tissue structure for regenerative medicine.
(© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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