Oral vitamin B 12 supplement is delivered to the distal gut, altering the corrinoid profile and selectively depleting Bacteroides in C57BL/6 mice.

Autor: Kelly CJ; Mucosal Inflammation Program, University of Colorado, Aurora, CO, USA.; Department of Medicine, University of Colorado, Aurora, CO, USA.; Department of Internal Medicine, Yale School of Medicine, New Haven, CT, USA., Alexeev EE; Mucosal Inflammation Program, University of Colorado, Aurora, CO, USA.; Department of Medicine, University of Colorado, Aurora, CO, USA., Farb L; Department of Medicine, University of Colorado, Aurora, CO, USA., Vickery TW; Department of Medicine, University of Colorado, Aurora, CO, USA.; Department of Medicine, University of Colorado Microbiome Research Consortium, Aurora, CO, USA., Zheng L; Mucosal Inflammation Program, University of Colorado, Aurora, CO, USA.; Department of Medicine, University of Colorado, Aurora, CO, USA., Eric L C; Mucosal Inflammation Program, University of Colorado, Aurora, CO, USA.; Department of Medicine, University of Colorado, Aurora, CO, USA.; Centre for Experimental Medicine, Queens University, Belfast, UK., Kitzenberg DA; Mucosal Inflammation Program, University of Colorado, Aurora, CO, USA.; Department of Medicine, University of Colorado, Aurora, CO, USA., Battista KD; Mucosal Inflammation Program, University of Colorado, Aurora, CO, USA.; Department of Medicine, University of Colorado, Aurora, CO, USA., Kominsky DJ; Mucosal Inflammation Program, University of Colorado, Aurora, CO, USA.; Centre for Experimental Medicine, Queens University, Belfast, UK.; Department of Microbiology and Immunology, Montana State University, Bozeman, MT, USA., Robertson CE; Department of Medicine, University of Colorado, Aurora, CO, USA.; Department of Medicine, University of Colorado Microbiome Research Consortium, Aurora, CO, USA., Frank DN; Department of Medicine, University of Colorado, Aurora, CO, USA.; Department of Medicine, University of Colorado Microbiome Research Consortium, Aurora, CO, USA., Stabler SP; Department of Medicine, University of Colorado, Aurora, CO, USA., Colgan SP; Mucosal Inflammation Program, University of Colorado, Aurora, CO, USA.; Department of Medicine, University of Colorado, Aurora, CO, USA.
Jazyk: angličtina
Zdroj: Gut microbes [Gut Microbes] 2019; Vol. 10 (6), pp. 654-662. Date of Electronic Publication: 2019 May 07.
DOI: 10.1080/19490976.2019.1597667
Abstrakt: Vitamin B 12 is a critical nutrient for humans as well as microbes. Due to saturable uptake, high dose oral B 12 supplements are largely unabsorbed and reach the distal gut where they are available to interact with the microbiota. The aim of this study was to determine if oral B 12 supplementation in mice alters 1) the concentration of B 12 and related corrinoids in the distal gut, 2) the fecal microbiome, 3) short chain fatty acids (SCFA), and 4) susceptibility to experimental colitis. C57BL/6 mice (up to 24 animals/group) were supplemented with oral 3.94 µg/ml cyanocobalamin (B 12 ), a dose selected to approximate a single 5 mg supplement for a human. Active vitamin B 12 (cobalamin), and four B 12 -analogues ([ADE]CN-Cba, [2Me-ADE]CN-Cba, [2MeS-ADE]CN-Cba, CN-Cbi) were analyzed in cecal and fecal contents using liquid chromatography/mass spectrometry (LC/MS), in parallel with evaluation of fecal microbiota, cecal SCFA, and susceptibility to dextran sodium sulfate (DSS) colitis. At baseline, active B 12 was a minor constituent of overall cecal (0.86%) and fecal (0.44%) corrinoid. Oral B 12 supplementation increased active B 12 at distal sites by >130-fold (cecal B 12 increased from 0.08 to 10.60 ng/mg, fecal B 12 increased from 0.06 to 7.81 ng/ml) and reduced microbe-derived fecal corrinoid analogues ([ADE]CN-Cba, [2Me-ADE]CN-Cba, [2MeS-ADE]CN-Cba). Oral B 12 had no effect on cecal SCFA. Microbial diversity was unaffected by this intervention, however a selective decrease in Bacteroides was observed with B 12 treatment. Lastly, no difference in markers of DSS-induced colitis were detected with B 12 treatment.
Databáze: MEDLINE