The response to the DNA damaging agent methyl methanesulfonate in a fungal plant pathogen.

Autor: Milo-Cochavi S; Department of Plant Pathology and Microbiology, Hebrew University, Rehovot, 7610001, Israel., Pareek M; Department of Plant Pathology and Microbiology, Hebrew University, Rehovot, 7610001, Israel., Delulio G; Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA 01003, USA., Almog Y; Department of Plant Pathology and Microbiology, Hebrew University, Rehovot, 7610001, Israel., Anand G; Department of Plant Pathology and Microbiology, Hebrew University, Rehovot, 7610001, Israel., Ma LJ; Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA 01003, USA., Covo S; Department of Plant Pathology and Microbiology, Hebrew University, Rehovot, 7610001, Israel. Electronic address: shay.covo@mail.huji.ac.il.
Jazyk: angličtina
Zdroj: Fungal biology [Fungal Biol] 2019 May; Vol. 123 (5), pp. 408-422. Date of Electronic Publication: 2019 Apr 06.
DOI: 10.1016/j.funbio.2019.03.007
Abstrakt: DNA damage can cause mutations that in fungal plant pathogens lead to hypervirulence and resistance to pesticides. Almost nothing is known about the response of these fungi to DNA damage. We performed transcriptomic and phosphoproteomic analyses of Fusarium oxysporum exposed to methyl methanesulfonate (MMS). At the RNA level we observe massive induction of DNA repair pathways including the global genome nucleotide excision. Cul3, Cul4, several Ubiquitin-like ligases and components of the proteasome are significantly induced. In agreement, we observed drug synergism between a proteasome inhibitor and MMS. While our data suggest that Yap1 and Xbp1 networks are similarly activated in response to damage in yeast and F. oxysporum we were able to observe modules that were MMS-responsive in F. oxysporum and not in yeast. These include transcription/splicing modules that are upregulated and respiration that is down-regulated. In agreement, MMS treated cells are much more sensitive to a respiration inhibitor. At the phosphoproteomic level, Adenylate cyclase, which generates cAMP, is phosphorylated in response to MMS and forms a network of phosphorylated proteins that include cell cycle regulators and several MAPKs. Our analysis provides a starting point in understanding how genomic changes in response to DNA damage occur in Fusarium species.
(Copyright © 2019 British Mycological Society. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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