C9orf72 and triplet repeat disorder RNAs: G-quadruplex formation, binding to PRC2 and implications for disease mechanisms.

Autor:	Wang X; Department of Biochemistry, University of Colorado Boulder, Boulder, Colorado 80309, USA.; BioFrontiers Institute and Howard Hughes Medical Institute, University of Colorado Boulder, Boulder, Colorado 80309, USA., Goodrich KJ; Department of Biochemistry, University of Colorado Boulder, Boulder, Colorado 80309, USA.; BioFrontiers Institute and Howard Hughes Medical Institute, University of Colorado Boulder, Boulder, Colorado 80309, USA., Conlon EG; Department of Biological Sciences, Columbia University, New York, New York 10027, USA., Gao J; Department of Biological Sciences, Columbia University, New York, New York 10027, USA., Erbse AH; Department of Biochemistry, University of Colorado Boulder, Boulder, Colorado 80309, USA., Manley JL; Department of Biological Sciences, Columbia University, New York, New York 10027, USA., Cech TR; Department of Biochemistry, University of Colorado Boulder, Boulder, Colorado 80309, USA.; BioFrontiers Institute and Howard Hughes Medical Institute, University of Colorado Boulder, Boulder, Colorado 80309, USA.
Jazyk:	angličtina
Zdroj:	RNA (New York, N.Y.) [RNA] 2019 Aug; Vol. 25 (8), pp. 935-947. Date of Electronic Publication: 2019 May 02.
DOI:	10.1261/rna.071191.119
Abstrakt:	Some neurological disorders, including amyotrophic lateral sclerosis (ALS), frontotemporal dementia (FTD), fragile X syndrome, Huntington's disease, myotonic dystrophy, and various ataxias, can be caused by expansions of short nucleic acid sequence repeats in specific genes. A possible disease mechanism involves the transcribed repeat RNA binding an RNA-binding protein (RBP), resulting in its sequestration and thus dysfunction. Polycomb repressive complex 2 (PRC2), the histone methyltransferase that deposits the H3K27me3 mark of epigenetically silenced chromatin, binds G-rich RNAs and has especially high affinity for G-quadruplex (G-Q) structures. Here, we find that PRC2 target genes are derepressed and the RNA binding subunit EZH2 largely insoluble in postmortem brain samples from ALS/FTD patients with C9ORF72 (C9) repeat expansions, leading to the hypothesis that the (G 4 C 2 ) n repeat RNA might be sequestering PRC2. Contrary to this expectation, we found that C9 repeat RNAs ( n = 6 or 10) bind weakly to purified PRC2, and studies with the G-Q specific BG4 antibody and circular dichroism studies both indicated that these C9 RNAs have little propensity to form G-Qs in vitro. Several GC-rich triplet-repeat expansion RNAs also have low affinity for PRC2 and do not appreciably form G-Qs in vitro. The results are consistent with these sequences forming hairpin structures that outcompete G-Q folding when the repeat length is sufficiently large. We suggest that binding of PRC2 to these GC-rich RNAs is fundamentally weak but may be modulated in vivo by protein factors that affect secondary structure, such as helicases and other RBPs. (© 2019 Wang et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society.)
Databáze:	MEDLINE
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