Effect of cryoprotectant type and concentration on the vitrification of collared peccary (Pecari tajacu) ovarian tissue.

Autor: Lima GL; Laboratory on Animal Germplasm Conservation, Universidade Federal Rural do Semi-Árido - UFERSA, BR 110, Km 47, Costa e Silva, 59625-900, Mossoró, RN, Brazil., Luz VB; Laboratory on Animal Germplasm Conservation, Universidade Federal Rural do Semi-Árido - UFERSA, BR 110, Km 47, Costa e Silva, 59625-900, Mossoró, RN, Brazil., Lunardi FO; Laboratory of Manipulation of Oocytes and Preantral Follicles, Universidade Estadual do Ceará - UECE, Paranjana Ave, 1700, Itaperi, 60740-000, Fortaleza, CE, Brazil., Souza ALP; Laboratory on Animal Germplasm Conservation, Universidade Federal Rural do Semi-Árido - UFERSA, BR 110, Km 47, Costa e Silva, 59625-900, Mossoró, RN, Brazil., Peixoto GCX; Laboratory on Animal Germplasm Conservation, Universidade Federal Rural do Semi-Árido - UFERSA, BR 110, Km 47, Costa e Silva, 59625-900, Mossoró, RN, Brazil., Rodrigues APR; Laboratory of Manipulation of Oocytes and Preantral Follicles, Universidade Estadual do Ceará - UECE, Paranjana Ave, 1700, Itaperi, 60740-000, Fortaleza, CE, Brazil., Oliveira MF; Laboratory on Animal Germplasm Conservation, Universidade Federal Rural do Semi-Árido - UFERSA, BR 110, Km 47, Costa e Silva, 59625-900, Mossoró, RN, Brazil., Santos RR; Laboratory of Wild Animal Biology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Belém, Pará, Brazil; Schothorst Feed Research, the Netherlands., Silva AR; Laboratory on Animal Germplasm Conservation, Universidade Federal Rural do Semi-Árido - UFERSA, BR 110, Km 47, Costa e Silva, 59625-900, Mossoró, RN, Brazil. Electronic address: alexrs@ufersa.edu.br.
Jazyk: angličtina
Zdroj: Animal reproduction science [Anim Reprod Sci] 2019 Jun; Vol. 205, pp. 126-133. Date of Electronic Publication: 2019 Apr 26.
DOI: 10.1016/j.anireprosci.2019.04.012
Abstrakt: The aim of the present study was to establish a protocol for solid surface vitrification of peccary ovarian tissue by using different cryoprotectants. Ovarian pairs from five adult females were fragmented and two fragments (fresh control group) were immediately subjected to morphological evaluation using classical histology, transmission electron microscopy, and viability analysis using fluorescent probes. The remaining fragments (n = 18) were vitrified using a solid surface method with different concentrations (3 or 6 M) of ethylene glycol (EG), dimethyl sulfoxide (DMSO) or dimethyl formamide (DMF). After 2 weeks, samples were re-warmed and evaluated. A decrease in the percentage of morphologically normal preantral follicles (PFs) was verified for all the groups in comparison to the fresh control (92.0 ± 2.8%); however, if only the primordial follicles are considered, the most effective preservation (P < 0.05) was achieved with the use of EG at 3 M (74.2±7.3%) or DMSO at 6 M (75.0 ± 4.2%). Ultrastructural analysis indicated there were well-preserved PFs in all the groups evaluated, having well-defined membranes, a few vacuoles, and organelles that were uniformly distributed throughout the cytoplasm, mainly round and elongated mitochondria in close association with lipid droplets. Viability was preserved (P <  0.05) with the use of EG at 3 (97%) or 6 (97%) M, DMSO at 3 (100%), and DMF at 6 (97%) M. Solid surface vitrification, therefore, is an effective method for conservation of peccary female germplasm, especially with the use of EG at 3 M, which was highly effective for preservation of both the morphology and viability of PFs.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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