Binding interaction study on human serum albumin with bactericidal gold nanoparticles synthesized from a leaf extract of Musa balbisiana: a multispectroscopic approach.
Autor: | Maji A; Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, West Bengal, India., Beg M; Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, West Bengal, India., Das S; Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, West Bengal, India., Sahoo NK; Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, West Bengal, India., Jha PK; School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, West Bengal, India., Islam MM; Department of Chemistry, Aliah University, West Bengal, India., Hossain M; Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, West Bengal, India. |
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Jazyk: | angličtina |
Zdroj: | Luminescence : the journal of biological and chemical luminescence [Luminescence] 2019 Sep; Vol. 34 (6), pp. 563-575. Date of Electronic Publication: 2019 May 01. |
DOI: | 10.1002/bio.3639 |
Abstrakt: | This study describes the eco-friendly, low-cost and room-temperature synthesis of gold nanoparticles from Musa balbisiana leaf extract, which acts as both reducing and stabilizing agent, and characterized by ultraviolet-visible (UV-vis) light spectroscopy, fourier transform infrared (FTIR) spectroscopy, field emission scanning electron microscopy (FE-SEM), analytical transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopy (EDAX) and dynamic light scattering (DLS) instruments. These nanoparticles showed an average diameter of 33.83 ± 3.39 nm, which was confirmed from the size distribution histogram. The bactericidal activity of these nanoparticles was confirmed using bacteria Escherichia coli and Staphylococcus aureus at 1 and 2 nM minimum inhibitory concentrations, respectively. The interaction between nanoparticles and human serum albumin (HSA) was investigated, as this plays significant roles in biological systems. The nature of interaction, binding parameters and structural variation of HSA in the presence of these nanoparticles have been evaluated using several useful spectroscopic approaches such as UV-vis, FTIR, time-resolved and steady-state fluorescence, and circular dichroism in addition to the measurement of zeta potential. This interaction study revealed that static quenching occurs in this process with minimal alteration in the secondary structure, but the native structure of HSA remained unaltered. The binding constant and thermodynamic parameters of this interaction process were also evaluated. (© 2019 John Wiley & Sons, Ltd.) |
Databáze: | MEDLINE |
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