PCGF6 regulates stem cell pluripotency as a transcription activator via super-enhancer dependent chromatin interactions.

Autor: Huang X; RNA Biomedical Institute, Sun Yat-Sen Memorial Hospital, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510080, China.; Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-Sen University, Guangzhou, 510080, China.; Department of Cell Biology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China.; Program in Stem Cell and Regenerative Medicine, The Third Affiliated Hospital of Sun Yat-sen University, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, China., Wei C; RNA Biomedical Institute, Sun Yat-Sen Memorial Hospital, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510080, China.; Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-Sen University, Guangzhou, 510080, China.; Department of Cell Biology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China.; Program in Stem Cell and Regenerative Medicine, The Third Affiliated Hospital of Sun Yat-sen University, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, China., Li F; RNA Biomedical Institute, Sun Yat-Sen Memorial Hospital, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510080, China.; Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-Sen University, Guangzhou, 510080, China.; Department of Cell Biology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China.; Program in Stem Cell and Regenerative Medicine, The Third Affiliated Hospital of Sun Yat-sen University, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, China., Jia L; Center for Bioinformatics, School of Life Sciences, Peking University, Beijing, 100871, China., Zeng P; RNA Biomedical Institute, Sun Yat-Sen Memorial Hospital, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510080, China.; Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-Sen University, Guangzhou, 510080, China.; Department of Cell Biology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China., Li J; RNA Biomedical Institute, Sun Yat-Sen Memorial Hospital, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510080, China.; Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-Sen University, Guangzhou, 510080, China.; Department of Cell Biology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China., Tan J; RNA Biomedical Institute, Sun Yat-Sen Memorial Hospital, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510080, China.; Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-Sen University, Guangzhou, 510080, China.; Department of Cell Biology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China., Sun T; RNA Biomedical Institute, Sun Yat-Sen Memorial Hospital, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510080, China.; Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-Sen University, Guangzhou, 510080, China.; Department of Cell Biology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China., Jiang S; RNA Biomedical Institute, Sun Yat-Sen Memorial Hospital, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510080, China.; Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-Sen University, Guangzhou, 510080, China.; Department of Cell Biology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China., Wang J; RNA Biomedical Institute, Sun Yat-Sen Memorial Hospital, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510080, China.; Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-Sen University, Guangzhou, 510080, China.; Department of Cell Biology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China., Tang X; RNA Biomedical Institute, Sun Yat-Sen Memorial Hospital, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510080, China.; Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-Sen University, Guangzhou, 510080, China.; Department of Cell Biology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China., Zhao Q; RNA Biomedical Institute, Sun Yat-Sen Memorial Hospital, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510080, China.; Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-Sen University, Guangzhou, 510080, China.; Department of Cell Biology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China., Liu B; Department of Spine Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510630, China., Rong L; Department of Spine Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510630, China., Li C; Center for Bioinformatics, School of Life Sciences, Peking University, Beijing, 100871, China., Ding J; RNA Biomedical Institute, Sun Yat-Sen Memorial Hospital, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510080, China. dingjunj@mail.sysu.edu.cn.; Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Sun Yat-Sen University, Guangzhou, 510080, China. dingjunj@mail.sysu.edu.cn.; Department of Cell Biology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China. dingjunj@mail.sysu.edu.cn.; Program in Stem Cell and Regenerative Medicine, The Third Affiliated Hospital of Sun Yat-sen University, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, China. dingjunj@mail.sysu.edu.cn.; Department of Histology and Embryology, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, 511436, China. dingjunj@mail.sysu.edu.cn.
Jazyk: angličtina
Zdroj: Protein & cell [Protein Cell] 2019 Oct; Vol. 10 (10), pp. 709-725. Date of Electronic Publication: 2019 Apr 30.
DOI: 10.1007/s13238-019-0629-9
Abstrakt: Polycomb group (PcG) ring finger protein 6 (PCGF6), though known as a member of the transcription-repressing complexes, PcG, also has activation function in regulating pluripotency gene expression. However, the mechanism underlying the activation function of PCGF6 is poorly understood. Here, we found that PCGF6 co-localizes to gene activation regions along with pluripotency factors such as OCT4. In addition, PCGF6 was recruited to a subset of the super-enhancer (SE) regions upstream of cell cycle-associated genes by OCT4, and increased their expression. By combining with promoter capture Hi-C data, we found that PCGF6 activates cell cycle genes by regulating SE-promoter interactions via 3D chromatin. Our findings highlight a novel mechanism of PcG protein in regulating pluripotency, and provide a research basis for the therapeutic application of pluripotent stem cells.
Databáze: MEDLINE
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