| Autor: |
Montagne J; Sección Biología Celular, Facultad de Ciencias, Universidad de la República, Iguá 4225, CP11400, Montevideo, Uruguay., Preza M; Sección Biología Celular, Facultad de Ciencias, Universidad de la República, Iguá 4225, CP11400, Montevideo, Uruguay., Castillo E; Sección Bioquímica, Facultad de Ciencias, Universidad de la República, Iguá 4225, CP11400, Montevideo, Uruguay., Brehm K; Institute of Hygiene and Microbiology, University of Würzburg, Josef-Schneider-Straße 2, 97080, Würzburg, Germany., Koziol U; Sección Biología Celular, Facultad de Ciencias, Universidad de la República, Iguá 4225, CP11400, Montevideo, Uruguay. ukoziol@gmail.com. |
| Abstrakt: |
The Wnt/beta-catenin pathway has many key roles in the development of animals, including a conserved and central role in the specification of the primary (antero-posterior) body axis. The posterior expression of Wnt ligands and the anterior expression of secreted Wnt inhibitors are known to be conserved during the larval metamorphosis of tapeworms. However, their downstream signaling components for Wnt/beta-catenin signaling have not been characterized. In this work, we have studied the core components of the beta-catenin destruction complex of the human pathogen Echinococcus multilocularis, the causative agent of alveolar echinococcosis. We focused on two Axin paralogs that are conserved in tapeworms and other flatworm parasites. Despite their divergent sequences, both Axins could robustly interact with one E. multilocularis beta-catenin paralog and limited its accumulation in a heterologous mammalian expression system. Similarly to what has been described in planarians (free-living flatworms), other beta-catenin paralogs showed limited or no interaction with either Axin and are unlikely to function as effectors in Wnt signaling. Additionally, both Axins interacted with three divergent GSK-3 paralogs that are conserved in free-living and parasitic flatworms. Axin paralogs have highly segregated expression patterns along the antero-posterior axis in the tapeworms E. multilocularis and Hymenolepis microstoma, indicating that different beta-catenin destruction complexes may operate in different regions during their larval metamorphosis. |
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