Temporal correlation of elevated PRMT1 gene expression with mushroom body neurogenesis during bumblebee brain development.

Autor: Guan C; School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, UK., Egertová M; School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, UK., Perry CJ; School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, UK., Chittka L; School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, UK., Chittka A; School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E1 4NS, UK. Electronic address: a.chittka@qmul.ac.uk.
Jazyk: angličtina
Zdroj: Journal of insect physiology [J Insect Physiol] 2019 Jul; Vol. 116, pp. 57-69. Date of Electronic Publication: 2019 Apr 27.
DOI: 10.1016/j.jinsphys.2019.04.011
Abstrakt: Neural development depends on the controlled proliferation and differentiation of neural precursors. In holometabolous insects, these processes must be coordinated during larval and pupal development. Recently, protein arginine methylation has come into focus as an important mechanism of controlling neural stem cell proliferation and differentiation in mammals. Whether a similar mechanism is at work in insects is unknown. We investigated this possibility by determining the expression pattern of three protein arginine methyltransferase mRNAs (PRMT1, 4 and 5) in the developing brain of bumblebees by in situ hybridisation. We detected expression in neural precursors and neurons in functionally important brain areas throughout development. We found markedly higher expression of PRMT1, but not PRMT4 and PRMT5, in regions of mushroom bodies containing dividing cells during pupal stages at the time of active neurogenesis within this brain area. At later stages of development, PRMT1 expression levels were found to be uniform and did not correlate with actively dividing cells. Our study suggests a role for PRMT1 in regulating neural precursor divisions in the mushroom bodies of bumblebees during the period of neurogenesis.
(Copyright © 2019 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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