| Autor: |
Zhou X; College of Animal Science, Jilin University, 5333 Xi An Road, Changchun 130062, Jilin, China. xiaolu_zhou0416@163.com., Wu S; College of Basic Medical Sciences, Jilin University, 126 Xin Min Avenue, Changchun 130021, Jilin, China. slwu@jlu.edu.cn., Zhou H; College of Animal Science, Jilin University, 5333 Xi An Road, Changchun 130062, Jilin, China. hongdazhou521@163.com., Wang M; College of Animal Science, Jilin University, 5333 Xi An Road, Changchun 130062, Jilin, China. mengyun_wang1124@163.com., Wang M; College of Animal Science, Jilin University, 5333 Xi An Road, Changchun 130062, Jilin, China. menghanwang927@163.com., Lü Y; College of Animal Science, Jilin University, 5333 Xi An Road, Changchun 130062, Jilin, China. lvyanty@jlu.edu.cn., Cheng Z; Jingjie PTM Biolabs Co. Ltd., 452 6th Street, Hangzhou Eco. & Tech. Developmental Area, Hangzhou 310018, Zhejiang, China. zhongyi_cheng@ptm-biolab.com., Xu J; College of Animal Science, Jilin University, 5333 Xi An Road, Changchun 130062, Jilin, China. jcxu@jlu.edu.cn., Ai Y; College of Animal Science, Jilin University, 5333 Xi An Road, Changchun 130062, Jilin, China. aiyx@jlu.edu.cn. |
| Abstrakt: |
Ubiquitination and deubiquitination of cellular proteins are reciprocal reactions catalyzed by ubiquitination-related enzymes and deubiquitinase (DUB) which regulate almost all cellular processes. Marek's disease virus (MDV) encodes a viral DUB that plays an important role in the MDV pathogenicity. Chicken CD4 + T-cell lymphoma induced by MDV is a key contributor to multiple visceral tumors and immunosuppression of chickens with Marek's disease (MD). However, alterations in the ubiquitylome of MDV-induced T lymphoma cells are still unclear. In this study, a specific antibody against K-ε-GG was used to isolate ubiquitinated peptides from CD4 + T cells and MD T lymphoma cells. Mass spectrometry was used to compare and analyze alterations in the ubiquitylome. Our results showed that the ubiquitination of 717 and 778 proteins was significantly up- and downregulated, respectively, in T lymphoma cells. MDV up- and downregulated ubiquitination of a similar percentage of proteins. The ubiquitination of transferases, especially serine/threonine kinases, was the main regulatory target of MDV. Compared with CD4 + T cells of the control group, MDV mainly altered the ubiquitylome associated with the signal transduction, immune system, cancer, and infectious disease pathways in T lymphoma cells. In these pathways, the ubiquitination of CDK1, IL-18, PRKCB, ETV6, and EST1 proteins was significantly up- or downregulated as shown by immunoblotting. The current study revealed that the MDV infection could exert a significant influence on the ubiquitylome of CD4 + T cells. |
