Looking beyond the skin: Cutaneous and systemic oxidative stress in UVB-induced squamous cell carcinoma in hairless mice.

Autor: Carrara IM; Laboratory of Molecular Pathology, Londrina State University (UEL), Londrina, Paraná, Brazil, UEL, Rod. Celso Garcia Cid, PR-445, km 380, 86051-990 Londrina, Paraná, Brazil; Laboratory of Pathophysiology and Free Radicals, Londrina State University (UEL), Londrina, Paraná, Brazil, UEL, Rod. Celso Garcia Cid, PR-445, km 380, 86051-990 Londrina, Paraná, Brazil., Melo GP; Laboratory of Molecular Pathology, Londrina State University (UEL), Londrina, Paraná, Brazil, UEL, Rod. Celso Garcia Cid, PR-445, km 380, 86051-990 Londrina, Paraná, Brazil; Laboratory of Pathophysiology and Free Radicals, Londrina State University (UEL), Londrina, Paraná, Brazil, UEL, Rod. Celso Garcia Cid, PR-445, km 380, 86051-990 Londrina, Paraná, Brazil., Bernardes SS; Laboratory of Molecular Pathology, Londrina State University (UEL), Londrina, Paraná, Brazil, UEL, Rod. Celso Garcia Cid, PR-445, km 380, 86051-990 Londrina, Paraná, Brazil; Laboratory of Healthy Sciences Research, Federal University of Grande Dourados (UFGD), Dourados, Mato Grosso do Sul, Brazil, UFGD, R. João Rosa Góes, 1761 - Vila Progresso, Dourados, MS, 79825-070, Brazil. Electronic address: sarabernardes@ufgd.edu.br., Neto FS; Laboratory of Molecular Pathology, Londrina State University (UEL), Londrina, Paraná, Brazil, UEL, Rod. Celso Garcia Cid, PR-445, km 380, 86051-990 Londrina, Paraná, Brazil; Laboratory of Pathophysiology and Free Radicals, Londrina State University (UEL), Londrina, Paraná, Brazil, UEL, Rod. Celso Garcia Cid, PR-445, km 380, 86051-990 Londrina, Paraná, Brazil., Ramalho LNZ; Department of Pathology, Ribeirão Preto Medical School (FMRP), University of São Paulo (USP), FMRP, Av. Bandeirantes, 3900 - Monte Alegre, Ribeirão Preto, SP, 14049-900, Brazil. Electronic address: lramalho@fmrp.usp.br., Marinello PC; Laboratory of Molecular Pathology, Londrina State University (UEL), Londrina, Paraná, Brazil, UEL, Rod. Celso Garcia Cid, PR-445, km 380, 86051-990 Londrina, Paraná, Brazil., Luiz RC; Laboratory of Molecular Pathology, Londrina State University (UEL), Londrina, Paraná, Brazil, UEL, Rod. Celso Garcia Cid, PR-445, km 380, 86051-990 Londrina, Paraná, Brazil., Cecchini R; Laboratory of Pathophysiology and Free Radicals, Londrina State University (UEL), Londrina, Paraná, Brazil, UEL, Rod. Celso Garcia Cid, PR-445, km 380, 86051-990 Londrina, Paraná, Brazil., Cecchini AL; Laboratory of Molecular Pathology, Londrina State University (UEL), Londrina, Paraná, Brazil, UEL, Rod. Celso Garcia Cid, PR-445, km 380, 86051-990 Londrina, Paraná, Brazil. Electronic address: alcecchini@yahoo.com.
Jazyk: angličtina
Zdroj: Journal of photochemistry and photobiology. B, Biology [J Photochem Photobiol B] 2019 Jun; Vol. 195, pp. 17-26. Date of Electronic Publication: 2019 Apr 16.
DOI: 10.1016/j.jphotobiol.2019.04.007
Abstrakt: Cumulative ultraviolet (UV) exposure is associated with squamous skin cell carcinoma. UV radiation induces oxidative modifications in biomolecules of the skin leading to photocarcinogenesis. Indeed, the cyclobutene pyrimidine dimers and other dimers formed by photoaddition between carbon-carbon bonds also have an important role in the initiation process. However, information on the systemic redox status during these processes is scarce. Thus, we investigated the systemic redox profile in UVB-induced squamous cell carcinoma in mice. Female hairless mice were exposed to UVB radiation (cumulative dose = 17.1 J/cm 2 ). The dorsal skin of these mice developed actinic keratosis (AK) and squamous cell carcinoma (SCC) and presented increased levels of oxidative and nitrosative stress biomarkers (4-hydroxy-2-nonenal and 3-nitrotyrosine), and decreased antioxidant defenses. Systemically, we observed the consumption of plasmatic antioxidant defenses and increased levels of advanced oxidized protein products (AOPP), an oxidative stress product derived from systemic inflammatory response. Taken together, our results indicate that UVB chronic irradiation leads not only to adjacent and tumoral oxidative stress in the skin, but it systemically is reflected through the blood. These new findings clarify some aspects of the pathogenesis of SCC and should assist in formulating better chemoprevention strategies, while avoiding additional primary SCC development and metastasis.
(Copyright © 2019. Published by Elsevier B.V.)
Databáze: MEDLINE
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