Silk fibroin scaffolds seeded with Wharton's jelly mesenchymal stem cells enhance re-epithelialization and reduce formation of scar tissue after cutaneous wound healing.

Autor: Millán-Rivero JE; Hematopoietic Transplant and Cellular Therapy Unit, Instituto Murciano de Investigación Biosanitaria-Arrixaca, Virgen de la Arrixaca University Hospital, University of Murcia, Murcia, Spain.; Internal Medicine Department, Medicine School, University of Murcia, Avenida Buenavista s/n. El Palmar, Murcia, Spain., Martínez CM; Experimental Pathology Unit, Instituto Murciano de Investigación Biosanitaria (IMIB)-Arrixaca, Murcia, Spain., Romecín PA; Hematopoietic Transplant and Cellular Therapy Unit, Instituto Murciano de Investigación Biosanitaria-Arrixaca, Virgen de la Arrixaca University Hospital, University of Murcia, Murcia, Spain., Aznar-Cervantes SD; Biotechnology Department, Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), Murcia, Spain., Carpes-Ruiz M; Experimental Pathology Unit, Instituto Murciano de Investigación Biosanitaria (IMIB)-Arrixaca, Murcia, Spain., Cenis JL; Biotechnology Department, Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), Murcia, Spain., Moraleda JM; Hematopoietic Transplant and Cellular Therapy Unit, Instituto Murciano de Investigación Biosanitaria-Arrixaca, Virgen de la Arrixaca University Hospital, University of Murcia, Murcia, Spain.; Internal Medicine Department, Medicine School, University of Murcia, Avenida Buenavista s/n. El Palmar, Murcia, Spain., Atucha NM; Hematopoietic Transplant and Cellular Therapy Unit, Instituto Murciano de Investigación Biosanitaria-Arrixaca, Virgen de la Arrixaca University Hospital, University of Murcia, Murcia, Spain.; Physiology Department, Medicine School, University of Murcia, Murcia, Spain., García-Bernal D; Hematopoietic Transplant and Cellular Therapy Unit, Instituto Murciano de Investigación Biosanitaria-Arrixaca, Virgen de la Arrixaca University Hospital, University of Murcia, Murcia, Spain. david.garcia23@um.es.; Internal Medicine Department, Medicine School, University of Murcia, Avenida Buenavista s/n. El Palmar, Murcia, Spain. david.garcia23@um.es.
Jazyk: angličtina
Zdroj: Stem cell research & therapy [Stem Cell Res Ther] 2019 Apr 27; Vol. 10 (1), pp. 126. Date of Electronic Publication: 2019 Apr 27.
DOI: 10.1186/s13287-019-1229-6
Abstrakt: Background: The treatment of extensive and/or chronic skin wounds is a widespread and costly public health problem. Mesenchymal stem cells (MSCs) have been proposed as a potential cell therapy for inducing wound healing in different clinical settings, alone or in combination with biosynthetic scaffolds. Among them, silk fibroin (SF) seeded with MSCs has been shown to have increased efficacy in skin wound healing experimental models.
Methods: In this report, we investigated the wound healing effects of electrospun SF scaffolds cellularized with human Wharton's jelly MSCs (Wj-MSCs-SF) using a murine excisional wound splinting model.
Results: Immunohistopathological examination after transplant confirmed the presence of infiltrated human fibroblast-like CD90-positive cells in the dermis of the Wj-MSCs-SF-treated group, yielding neoangiogenesis, decreased inflammatory infiltrate and myofibroblast proliferation, less collagen matrix production, and complete epidermal regeneration.
Conclusions: These findings indicate that Wj-MSCs transplanted in the wound bed on a silk fibroin scaffold contribute to the generation of a well-organized and vascularized granulation tissue, enhance reepithelization of the wound, and reduce the formation of fibrotic scar tissue, highlighting the potential therapeutic effects of Wj-MSC-based tissue engineering approaches to non-healing wound treatment.
Databáze: MEDLINE
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