Effect of Chitosan-(Poly)Nitroxides on Normal and Tumor Cells under Conditions of Induced Oxidative Stress.

Autor: Mumyatova VA; Institute of Problems Chemical Physics, Russian Academy of Sciences, Chernogolovka, Moscow region, Russia. vmumyatova@bk.ru.; P. G. Demidov Yaroslavl State University, Yaroslavl, Russia. vmumyatova@bk.ru.; Medical Chemistry Research and Education Center, Moscow Regional State University, Mytishchi, Moscow region, Russia. vmumyatova@bk.ru., Balakina AA; Institute of Problems Chemical Physics, Russian Academy of Sciences, Chernogolovka, Moscow region, Russia.; P. G. Demidov Yaroslavl State University, Yaroslavl, Russia.; Medical Chemistry Research and Education Center, Moscow Regional State University, Mytishchi, Moscow region, Russia., Sen' VD; Institute of Problems Chemical Physics, Russian Academy of Sciences, Chernogolovka, Moscow region, Russia.; P. G. Demidov Yaroslavl State University, Yaroslavl, Russia., Pliss EM; P. G. Demidov Yaroslavl State University, Yaroslavl, Russia., Terent'ev AA; Institute of Problems Chemical Physics, Russian Academy of Sciences, Chernogolovka, Moscow region, Russia.; P. G. Demidov Yaroslavl State University, Yaroslavl, Russia.; Medical Chemistry Research and Education Center, Moscow Regional State University, Mytishchi, Moscow region, Russia.; Faculty of Fundamental Physical and Chemical Engineering, M. V. Lomonosov Moscow State University, Moscow, Russia.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2019 Apr; Vol. 166 (6), pp. 779-784. Date of Electronic Publication: 2019 Apr 26.
DOI: 10.1007/s10517-019-04439-7
Abstrakt: The cytotoxicity and antioxidant effects of chitosan-(poly)nitoxides of different molecular weights containing a nitroxide radical of the piperidine structure were studied on tumor (HeLa, A172, and HepG2) and normal (Vero) cell lines. The chitosan-(poly)nitroxides exhibited low cytotoxicity. Under conditions of oxidative stress induced with tert-butyl hydroperoxide, the most pronounced decrease in ROS levels in the presence of chitosan-(poly)nitroxides was observed in normal cells. In cell homogenates, the decrease in malondialdehyde levels was observed only in the presence of low-molecular-weight chitosan-(poly)nitroxide irrespective of the cell line. Our data demonstrate that the cell-specific antioxidant properties of chitosan-(poly)nitroxides are related to their penetration into cells and interaction with intracellular membranes.
Databáze: MEDLINE
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