A fission yeast platform for heterologous expression of mammalian adenylyl cyclases and high throughput screening.
Autor: | Getz RA; Biology Department, Boston College, 140 Commonwealth Ave., Chestnut Hill, MA 02467, USA., Kwak G; Biology Department, Boston College, 140 Commonwealth Ave., Chestnut Hill, MA 02467, USA., Cornell S; Biology Department, Boston College, 140 Commonwealth Ave., Chestnut Hill, MA 02467, USA., Mbugua S; Biology Department, Boston College, 140 Commonwealth Ave., Chestnut Hill, MA 02467, USA. Electronic address: mbugua@bc.edu., Eberhard J; Biology Department, Boston College, 140 Commonwealth Ave., Chestnut Hill, MA 02467, USA. Electronic address: eberharj@bc.edu., Huang SX; Biology Department, Boston College, 140 Commonwealth Ave., Chestnut Hill, MA 02467, USA. Electronic address: huangsi@bc.edu., Abbasi Z; Biology Department, Boston College, 140 Commonwealth Ave., Chestnut Hill, MA 02467, USA. Electronic address: abbasiz@bc.edu., de Medeiros AS; Biology Department, Boston College, 140 Commonwealth Ave., Chestnut Hill, MA 02467, USA., Thomas R; Biology Department, Boston College, 140 Commonwealth Ave., Chestnut Hill, MA 02467, USA. Electronic address: thomasry@bc.edu., Bukowski B; Biology Department, Boston College, 140 Commonwealth Ave., Chestnut Hill, MA 02467, USA. Electronic address: bukowskb@bc.edu., Dranchak PK; National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, MD 20850, USA. Electronic address: patricia.dranchak@nih.gov., Inglese J; National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, MD 20850, USA. Electronic address: jinglese@mail.nih.gov., Hoffman CS; Biology Department, Boston College, 140 Commonwealth Ave., Chestnut Hill, MA 02467, USA. Electronic address: hoffmacs@bc.edu. |
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Jazyk: | angličtina |
Zdroj: | Cellular signalling [Cell Signal] 2019 Aug; Vol. 60, pp. 114-121. Date of Electronic Publication: 2019 Apr 24. |
DOI: | 10.1016/j.cellsig.2019.04.010 |
Abstrakt: | The fission yeast Schizosaccharomyces pombe uses a cAMP signaling pathway to link glucose-sensing to Protein Kinase A activity in order to regulate cell growth, sexual development, gluconeogenesis, and exit from stationary phase. We previously used a PKA-repressed fbp1-ura4 reporter to conduct high throughput screens (HTSs) for inhibitors of heterologously-expressed mammalian cyclic nucleotide phosphodiesterases (PDEs). Here, we describe the successful expression of all ten mammalian adenylyl cyclase (AC) genes, along with the human GNAS Gα (Copyright © 2019 Elsevier Inc. All rights reserved.) |
Databáze: | MEDLINE |
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