| Autor: |
Preena PG; National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Fine Arts Avenue, Cochin 682016, India E-mail: isbsingh@gmail.com., Achuthan C; National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Fine Arts Avenue, Cochin 682016, India E-mail: isbsingh@gmail.com., Kumar VJR; National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Fine Arts Avenue, Cochin 682016, India E-mail: isbsingh@gmail.com; Present address: Department of Aquaculture, Kerala University of Fisheries and Ocean Studies, Panangad, Kochi 682506, India., Boobal R; National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Fine Arts Avenue, Cochin 682016, India E-mail: isbsingh@gmail.com., Deepa GD; National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Fine Arts Avenue, Cochin 682016, India E-mail: isbsingh@gmail.com., Puthumana J; National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Fine Arts Avenue, Cochin 682016, India E-mail: isbsingh@gmail.com., Poulose S; National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Fine Arts Avenue, Cochin 682016, India E-mail: isbsingh@gmail.com., Surekhamol IS; National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Fine Arts Avenue, Cochin 682016, India E-mail: isbsingh@gmail.com., Singh ISB; National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Fine Arts Avenue, Cochin 682016, India E-mail: isbsingh@gmail.com. |
| Abstrakt: |
To mitigate the toxicity of ammonia in aquaculture systems, marine and brackish water ammonia-oxidizing bacterial consortia have been developed and are used for activation of nitrifying bioreactors integrated to recirculating aquaculture systems. To shed more light on to these biological entities, diversity of both the consortia were analyzed based on random cloning of 16S rRNA gene and ammonia-oxidizing bacterial specific amoA gene sequences. The dendrograms of representative clones on the basis of amplified ribosomal DNA restriction analysis generated 22 and 19 clusters for marine and brackish water nitrifying consortia, respectively. Phylogenetic analysis demonstrated the presence of various autotrophic nitrifiers belonging to α-, β- and γ-Proteobacteria, anaerobic ammonia oxidizers, heterotrophic denitrifiers, Bacteroidetes, and Actinobacteria. Distribution patterns of the organisms within the two consortia were determined using the software Geneious and diversity indices were investigated using Mega 5.0, VITCOMIC and Primer 7. The abundance of ammonia oxidizers was found in the order of 2.21 ± 0.25 × 10 9 copies/g wet weight of marine consortium and 6.20 ± 0.23 × 10 7 copies/g of brackish water consortium. Besides, marine ammonia-oxidizing consortium exhibited higher mean population diversity and Shannon Wiener diversity than the brackish water counterparts. |
