Pilot study to quantify the time to clear dexamethasone from plasma and urine of adult horses following a single nebulisation.

Autor: Symonds NE; Research and Clinical Trials Unit, University Veterinary Teaching Hospital Camden, University of Sydney, 410 Werombi Road, Camden, New South Wales, 2570, Australia., Dart AJ; Research and Clinical Trials Unit, University Veterinary Teaching Hospital Camden, University of Sydney, 410 Werombi Road, Camden, New South Wales, 2570, Australia., Keledjian J; Australian Racing Forensic Laboratory - Racing NSW, Sydney, NSW, Australia., Lau ML; Australian Racing Forensic Laboratory - Racing NSW, Sydney, NSW, Australia., Ennis LC; Australian Racing Forensic Laboratory - Racing NSW, Sydney, NSW, Australia., McIver VC; Research and Clinical Trials Unit, University Veterinary Teaching Hospital Camden, University of Sydney, 410 Werombi Road, Camden, New South Wales, 2570, Australia., Tsang AS; Research and Clinical Trials Unit, University Veterinary Teaching Hospital Camden, University of Sydney, 410 Werombi Road, Camden, New South Wales, 2570, Australia., Biasutti SA; Research and Clinical Trials Unit, University Veterinary Teaching Hospital Camden, University of Sydney, 410 Werombi Road, Camden, New South Wales, 2570, Australia., Jeffcott LB; Research and Clinical Trials Unit, University Veterinary Teaching Hospital Camden, University of Sydney, 410 Werombi Road, Camden, New South Wales, 2570, Australia.
Jazyk: angličtina
Zdroj: Australian veterinary journal [Aust Vet J] 2019 May; Vol. 97 (5), pp. 144-148.
DOI: 10.1111/avj.12800
Abstrakt: Objective: To quantify the time to clear dexamethasone from plasma and urine of horses following a single nebulisation.
Design: Experimental using six Standardbred mares.
Methods: Dexamethasone sodium phosphate (0.04 mg/kg) diluted in 0.9% sodium chloride was administered as an aerosol using a Flexineb E2® nebuliser. Blood samples (0, 2, 4, 6, 8, 10, 12, 24, 32, 48, 72 and 96 h) and urine samples (0, 1, 4, 8, 24, 32, 48, 72 and 96 h) were collected for analysis using liquid chromatography mass spectrometry.
Results: Maximum plasma concentrations (t max ) were reached by the earliest detection point (2 h) after nebulisation (0.6-1.8 ng/mL), but was no longer detectable at 48 h. However, in one horse 0.1 ng/mL was found at 96 h after three consecutive readings of 0 ng/mL. The t max in urine was reached by the earliest collection point (1 h) after nebulisation (3.2-23.8 ng/mL), but was no longer present in urine at 72 h in five horses, while detectable levels (0.1 ng/mL) were still present at 96 h in one horse.
Conclusions: A single dose of 0.04 mg/kg of DSP administered as an aerosol through a FlexinebE2® mask was no longer detectable in blood at 48 h in six horses tested, but one horse returned a reading of 0.1 ng/mL at 96 h after having no detectable levels. Dexamethasone was not detectable in urine at 72 h in five horses but was detectable at a low concentration (0.1 ng/mL) at 96 h in one horse.
(© 2019 Australian Veterinary Association.)
Databáze: MEDLINE
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