An Improved Screening Method for the Detection and Isolation of Escherichia coli 0157:H7 From Meat, Incorporating the 3M Petrifilm™ Test Kit - HEC - for Hemorrhagic Escherichia coli 0157:H7.

Autor:	Okrend AJG; Food Safety and Inspection Service, U.S. Department of Agriculture, Building 322, ARC-East, Beltsville, Maryland 20705., Rose BE; Food Safety and Inspection Service, U.S. Department of Agriculture, Building 322, ARC-East, Beltsville, Maryland 20705., Matner R; Microbiology Products, 3M Health Care, Bldg. 270-2N-04, 3M Center, St. Paul, Minnesota 55144.
Jazyk:	angličtina
Zdroj:	Journal of food protection [J Food Prot] 1990 Nov; Vol. 53 (11), pp. 936-940.
DOI:	10.4315/0362-028X-53.11.936
Abstrakt:	A screening method was devised incorporating a commercially available reactive disc blot ELISA for Escherichia coli 0157 antigen, into a cultural screening program for the isolation of E. coli 0157:H7 from meat and poultry products. The method includes the inoculation of a raw or cooked meat sample into an enrichment broth, incubation with shaking at 37°C for 6 to 8 h, followed by inoculation of 3M Petrifilm™ E. coli Count plates with dilutions of the enrichment culture. The Petrifilm plates were incubated at 42°C for 18 h and tested for the presence of the 0157 antigen. The enrichment cultures were reincubated static at 35°C after the initial shaken incubation. Isolation was attempted from the positive Petrifilm plates by both a direct picking and streaking method and by the 3M Prompt™ isolation method. Isolation also was attempted from the 24-h enrichment cultures by spread plating serial dilutions on 150 × 15 mm MacConkey sorbitol agar (MSA) and MSA with 5-bromo-4-chloro-3-indoxyl-β-D-glucuronic acid cyclohexylammonium salt (BCIG). This fast and efficient screening procedure identifies negative and presumptive positive samples in 26-28 h. Isolation and confirmation of the presumptive positive isolates require an additional 3 to 4 d.
Databáze:	MEDLINE
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