Engineering Escherichia coli for autoinducible production of L-valine: An example of an artificial positive feedback loop in amino acid biosynthesis.

Autor: Geraskina NV; Ajinomoto-Genetika Research Institute, Moscow, Russian Federation., Sycheva EV; Ajinomoto-Genetika Research Institute, Moscow, Russian Federation., Samsonov VV; Ajinomoto-Genetika Research Institute, Moscow, Russian Federation., Eremina NS; Ajinomoto-Genetika Research Institute, Moscow, Russian Federation., Hook CD; Ajinomoto-Genetika Research Institute, Moscow, Russian Federation., Serebrianyi VA; Ajinomoto-Genetika Research Institute, Moscow, Russian Federation., Stoynova NV; Ajinomoto-Genetika Research Institute, Moscow, Russian Federation.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2019 Apr 25; Vol. 14 (4), pp. e0215777. Date of Electronic Publication: 2019 Apr 25 (Print Publication: 2019).
DOI: 10.1371/journal.pone.0215777
Abstrakt: Artificial metabolically regulated inducible expression systems are often used for the production of essential compounds. In most cases, the application of such systems enables regulating the expression of an entire group of genes in response to any internal signal such as an aerobic/anaerobic switch, a transition to stationary phase, or the exhausting of essential compounds. In this work, we demonstrate an example of another type of artificial autoinducible module, denoted a positive feedback module. This positive feedback module generates an inducer molecule that in turn enhances its own synthesis, promoting an activation signal. Due to the use of acetolactate, an intermediate of the L-valine biosynthetic pathway, as a specific inducer molecule, we realized a positive feedback loop in the biosynthetic pathway of branched chain amino acids. Such positive feedback was demonstrated to improve the production of a target compound.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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