Profiling of Seed Proteome in Pea ( Pisum sativum L.) Lines Characterized with High and Low Responsivity to Combined Inoculation with Nodule Bacteria and Arbuscular Mycorrhizal Fungi.

Autor: Mamontova T; Department of Biochemistry, St. Petersburg State University, 199178 St. Petersburg, Russia. mamontova-bio@mail.ru.; Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany. mamontova-bio@mail.ru., Afonin AM; Department of Biotechnology, All-Russia Research Institute for Agricultural Microbiology, 196608 St. Petersburg, Russia. afoninalexeym@gmail.com., Ihling C; Department of Pharmaceutical Chemistry and Bioanalytics, Institute of Pharmacy, Martin-Luther Universität Halle-Wittenberg, 06120 Halle (Saale), Germany. christian.ihling@pharmazie.uni-halle.de., Soboleva A; Department of Biochemistry, St. Petersburg State University, 199178 St. Petersburg, Russia. oriselle@yandex.ru.; Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany. oriselle@yandex.ru., Lukasheva E; Department of Biochemistry, St. Petersburg State University, 199178 St. Petersburg, Russia. elena_lukasheva@mail.ru., Sulima AS; Department of Biotechnology, All-Russia Research Institute for Agricultural Microbiology, 196608 St. Petersburg, Russia. sulima_arriam@yandex.ru., Shtark OY; Department of Biotechnology, All-Russia Research Institute for Agricultural Microbiology, 196608 St. Petersburg, Russia. shtark_arriam@yandex.ru., Akhtemova GA; Department of Biotechnology, All-Russia Research Institute for Agricultural Microbiology, 196608 St. Petersburg, Russia. ahgulya@yandex.ru., Povydysh MN; R&D department, Saint-Petersburg State Chemical and Pharmaceutical University, 197376 St. Petersburg, Russia. maria.povydysh@pharminnotech.com., Sinz A; Department of Pharmaceutical Chemistry and Bioanalytics, Institute of Pharmacy, Martin-Luther Universität Halle-Wittenberg, 06120 Halle (Saale), Germany. andrea.sinz@pharmazie.uni-halle.de., Frolov A; Department of Biochemistry, St. Petersburg State University, 199178 St. Petersburg, Russia. afrolov@ipb-halle.de.; Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, 06120 Halle (Saale), Germany. afrolov@ipb-halle.de., Zhukov VA; Department of Biotechnology, All-Russia Research Institute for Agricultural Microbiology, 196608 St. Petersburg, Russia. vladimir.zhukoff@gmail.com., Tikhonovich IA; Department of Biotechnology, All-Russia Research Institute for Agricultural Microbiology, 196608 St. Petersburg, Russia. arriam2008@yandex.ru.; Department of Genetics and Biotechnology, St. Petersburg State University, 199034 St. Petersburg, Russia. arriam2008@yandex.ru.
Jazyk: angličtina
Zdroj: Molecules (Basel, Switzerland) [Molecules] 2019 Apr 23; Vol. 24 (8). Date of Electronic Publication: 2019 Apr 23.
DOI: 10.3390/molecules24081603
Abstrakt: Legume crops represent the major source of food protein and contribute to human nutrition and animal feeding. An essential improvement of their productivity can be achieved by symbiosis with beneficial soil microorganisms-rhizobia (Rh) and arbuscular mycorrhizal (AM) fungi. The efficiency of these interactions depends on plant genotype. Recently, we have shown that, after simultaneous inoculation with Rh and AM, the productivity gain of pea ( Pisum sativum L) line K-8274, characterized by high efficiency of interaction with soil microorganisms (EIBSM), was higher in comparison to a low-EIBSM line K-3358. However, the molecular mechanisms behind this effect are still uncharacterized. Therefore, here, we address the alterations in pea seed proteome, underlying the symbiosis-related productivity gain, and identify 111 differentially expressed proteins in the two lines. The high-EIBSM line K-8274 responded to inoculation by prolongation of seed maturation, manifested by up-regulation of proteins involved in cellular respiration, protein biosynthesis, and down-regulation of late-embryogenesis abundant (LEA) proteins. In contrast, the low-EIBSM line K-3358 demonstrated lower levels of the proteins, related to cell metabolism. Thus, we propose that the EIBSM trait is linked to prolongation of seed filling that needs to be taken into account in pulse crop breeding programs. The raw data have been deposited to the ProteomeXchange with identifier PXD013479.
Competing Interests: The authors declare no conflicts of interest.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje