Novel record of a tick Amblyomma sculptum with detection of piroplasm Rangelia vitalii DNA.

Autor: Campos SDE; Departamento de Patologia e Clínica Veterinária, Universidade Federal Fluminense, Niterói, RJ, Brazil. Electronic address: sabrinadec@id.uff.br., Toma HK; Laboratório de Diagnóstico Molecular e Hematologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil., Machado CSC; Departamento de Patologia e Clínica Veterinária, Universidade Federal Fluminense, Niterói, RJ, Brazil., Assad RQ; Departamento de Patologia e Clínica Veterinária, Universidade Federal Fluminense, Niterói, RJ, Brazil., Almosny NRP; Departamento de Patologia e Clínica Veterinária, Universidade Federal Fluminense, Niterói, RJ, Brazil.
Jazyk: angličtina
Zdroj: Veterinary parasitology, regional studies and reports [Vet Parasitol Reg Stud Reports] 2018 Aug; Vol. 13, pp. 228-229. Date of Electronic Publication: 2018 Jul 18.
DOI: 10.1016/j.vprsr.2018.07.007
Abstrakt: Rangelia vitalii is the protozoan responsible for a febrile tick-borne disease known as "Nambiuvú" or "bleeding plague." The only competent vector of this protozoan recognized in Brazil is the tick Amblyomma aureolatum. The aim of this study was to identify R. vitalii using molecular biology in ticks parasitizing Cerdocyon thous from Tijuca and Serra dos Órgãos National Parks, which are areas of the Atlantic Rainforest biome in the municipality of Rio de Janeiro. After taxonomic identification, ticks were crushed and DNA was extracted and examined by polymerase chain reaction (PCR) to verify amplification of a fragment of the 18S rRNA gene of piroplasms. Samples identified as positive in the PCR analysis were subjected to sequencing, which revealed high identity with sequences of R. vitalii. This is the first report of parasitizing nymphs of A. sculptum infected by R. vitalii under natural conditions.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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