Association Between miR-155 , Its Polymorphism and Ischemia-Modified Albumin in Patients with Rheumatoid Arthritis.

Autor: Shaker OG; 1 Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt., Abdelaleem OO; 2 Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Fayoum University, Faiyum, Egypt., Fouad NA; 3 Department of Rheumatology and Rehabilitation, Faculty of Medicine, Fayoum University, Faiyum, Egypt., Ali AMEA; 4 Department of Medical Physiology, Faculty of Medicine, Fayoum University, Faiyum, Egypt., Ahmed TI; 5 Department of Internal Medicine, Faculty of Medicine, Fayoum University, Faiyum, Egypt., Ibrahem EG; 6 Department of Medical Microbiology and Immunology, Faculty of Medicine, Fayoum University, Faiyum, Egypt., Abdelghaffar NK; 7 Department of Clinical Pathology, Faculty of Medicine, Fayoum University, Faiyum, Egypt.
Jazyk: angličtina
Zdroj: Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research [J Interferon Cytokine Res] 2019 Jul; Vol. 39 (7), pp. 428-437. Date of Electronic Publication: 2019 Apr 22.
DOI: 10.1089/jir.2019.0001
Abstrakt: Rheumatoid arthritis (RA) is a chronic immune-mediated inflammatory disease. We aimed to measure the level of miR-155 and its genetic variant rs767649 in patients with RA and to evaluate their relationship with ischemia-modified albumin (IMA). The study was performed on 79 patients with RA (group I) and 78 healthy control participants (group II). Quantitative real-time polymerase chain reaction was used to assess the expression of serum miR-155 in addition to its functional variant rs767649. IMA levels were measured by enzyme-linked immunosorbent assay. Significant overexpression of miR-155 and higher levels of IMA were detected in patients with RA compared with those in controls ( P  < 0.0001). The fold change in miR-155 was significantly positively associated with IMA ( r  = 0.362, P  = 0.001) in patients with RA. Significant differences in the frequency of miR-155 (rs767649) genotypes and alleles were noted between patients with RA and controls. MiR-155 and IMA levels were significantly associated with the genotype distribution of miR-155 (rs767649) in patients with RA and were higher in patients with the TT genotype. MiR-155 and its functional variant rs767649 might play an important role in susceptibility to the increased risk of RA, stressing the role of miR-155 as a therapeutic target in the treatment of RA. In addition, IMA levels were increased and correlated with miR-155 and its single nucleotide polymorphism rs767649 in Egyptian patients with RA.
Databáze: MEDLINE
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