| Autor: |
Naraki Y; Department of Frontier Health Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, 7-2-10 Higashiogu, Arakawa-ku, Tokyo 116-8551, Japan., Watanabe M; Department of Frontier Health Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University, 7-2-10 Higashiogu, Arakawa-ku, Tokyo 116-8551, Japan., Takeya K; Department of Veterinary Biochemistry, Faculty of Veterinary Medicine, Okayama University of Science, Imabari-shi, Ehime 794-8555, Japan. |
| Abstrakt: |
Rubratoxin A, a potent inhibitor of PP2A, is known to suppress smooth muscle contraction. The inhibitory role of PP2A in smooth muscle contraction is still unclear. In order to clarify the regulatory mechanisms of PP2A on vascular smooth muscle contractility, we examined the effects of rubratoxin A on the Ca 2+ -induced contraction of β-escin skinned carotid artery preparations from guinea pigs. Rubratoxin A at 1 µM and 10 µM significantly inhibited skinned carotid artery contraction at any Ca 2+ concentration. The data fitting to the Hill equation in [Ca 2+ ]-contraction relationship indicated that rubratoxin A decreased F max-Ca2+ and increased [Ca 2+ ] 50 , indices of Ca 2+ sensitivity for the force and myosin-actin interaction, respectively. These results suggest that PP2A inhibition causes downregulation of the myosin light chain phosphorylation and direct interference with myosin-actin interaction. |
