Preparation of Zirconium-89 Solutions for Radiopharmaceutical Purposes: Interrelation Between Formulation, Radiochemical Purity, Stability and Biodistribution.

Autor: Larenkov A; State Research Center-Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency, Zhivopisnaya str., bld. 46; 123182 Moscow, Russia. anton.larenkov@gmail.com.; Lomonosov Moscow State University, GSP-1, Leninskie Gory, 119991 Moscow, Russia. anton.larenkov@gmail.com., Bubenschikov V; State Research Center-Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency, Zhivopisnaya str., bld. 46; 123182 Moscow, Russia. bubenschikov2011@yandex.ru., Makichyan A; State Research Center-Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency, Zhivopisnaya str., bld. 46; 123182 Moscow, Russia. makbezh@gmail.com., Zhukova M; State Research Center-Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency, Zhivopisnaya str., bld. 46; 123182 Moscow, Russia. 9053522@mail.ru., Krasnoperova A; State Research Center-Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency, Zhivopisnaya str., bld. 46; 123182 Moscow, Russia. kalina188@mail.ru., Kodina G; State Research Center-Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency, Zhivopisnaya str., bld. 46; 123182 Moscow, Russia. gkodina@yandex.ru.
Jazyk: angličtina
Zdroj: Molecules (Basel, Switzerland) [Molecules] 2019 Apr 18; Vol. 24 (8). Date of Electronic Publication: 2019 Apr 18.
DOI: 10.3390/molecules24081534
Abstrakt: Zirconium-89 is a promising radionuclide for nuclear medicine. The aim of the present work was to find a suitable method for obtaining zirconium-89 solutions for radiopharmaceutical purposes. For this purpose, the ion exchange behavior of zirconium-89 solutions was studied. Radio-TLC (thin layer chromatography) and biodistribution studies were carried out to understand speciation of zirconium-89 complexes and their role in the development of new radiopharmaceuticals. Three methods of zirconium-89 isolation were studied using ZR (hydroxamate) and Chelex-100 resins. It was found that ZR-resin alone is not enough to obtain stable zirconium-89 formulations. An easy and effective method of reconstitution of [ 89 Zr]Zr-oxalate to [ 89 Zr]Zr-citrate using Chelex-100 resin was developed. Developed procedures allow obtaining [ 89 Zr]Zr-oxalate (in 0.1 M sodium oxalate solution) and [ 89 Zr]Zr-citrate (in 0.1-1.0 M sodium citrate solution). These solutions are perfectly suitable and convenient for radiopharmaceutical purposes. Our results prove [ 89 Zr]Zr-citrate to be advantageous over [ 89 Zr]Zr-oxalate. During evaluation of speciation of zirconium-89 complexes, a new TLC method was developed, since it was proved that there is no comprehensive method for analysis or zirconium-89 preparations. The new method provides valuable insights about the content of "active" ionic form of zirconium-89. The interrelation of the chromatographic behavior of zirconium-89 preparations and their biodistribution was studied.
Competing Interests: The authors declare no conflict of interest. The founding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results.
Databáze: MEDLINE
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