Nanosecond duration pulsed electric field together with formic acid triggers caspase-dependent apoptosis in pathogenic yeasts.
Autor: | Novickij V; Institute of High Magnetic Fields, Vilnius Gediminas Technical University, Naugarduko st. 41, 03227 Vilnius, Lithuania; Department of Electrical Engineering, Vilnius Gediminas Technical University, Naugarduko st. 41, 03227 Vilnius, Lithuania., Staigvila G; Institute of High Magnetic Fields, Vilnius Gediminas Technical University, Naugarduko st. 41, 03227 Vilnius, Lithuania; Department of Electrical Engineering, Vilnius Gediminas Technical University, Naugarduko st. 41, 03227 Vilnius, Lithuania., Gudiukaitė R; Department of Microbiology and Biotechnology, Institute of Biosciences, Center of Life Sciences, Vilnius University, Saulėtekio ave 7, 10257 Vilnius, Lithuania., Zinkevičienė A; Department of Immunology, State Research Institute Centre for Innovative Medicine, Santariškių st. 5, 08406 Vilnius, Lithuania., Girkontaitė I; Department of Immunology, State Research Institute Centre for Innovative Medicine, Santariškių st. 5, 08406 Vilnius, Lithuania., Paškevičius A; Laboratory of Biodeterioration Research, Nature Research Centre, Akademijos st. 2, 08412 Vilnius, Lithuania., Švedienė J; Laboratory of Biodeterioration Research, Nature Research Centre, Akademijos st. 2, 08412 Vilnius, Lithuania., Markovskaja S; Laboratory of Mycology, Nature Research Centre, Zaliuju ezeru st. 49, 08406 Vilnius, Lithuania., Novickij J; Institute of High Magnetic Fields, Vilnius Gediminas Technical University, Naugarduko st. 41, 03227 Vilnius, Lithuania; Department of Electrical Engineering, Vilnius Gediminas Technical University, Naugarduko st. 41, 03227 Vilnius, Lithuania; Institute of Telecommunications, Vilnius Gediminas Technical University, Naugarduko st. 41, 03227, Vilnius, Lithuania., Lastauskienė E; Department of Microbiology and Biotechnology, Institute of Biosciences, Center of Life Sciences, Vilnius University, Saulėtekio ave 7, 10257 Vilnius, Lithuania. Electronic address: egle.lastauskiene@gf.vu.lt. |
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Jazyk: | angličtina |
Zdroj: | Bioelectrochemistry (Amsterdam, Netherlands) [Bioelectrochemistry] 2019 Aug; Vol. 128, pp. 148-154. Date of Electronic Publication: 2019 Apr 09. |
DOI: | 10.1016/j.bioelechem.2019.04.007 |
Abstrakt: | Antifungal substances that are used for the treatment of candidiasis have considerable side effects and Candida yeasts are known to obtain drug resistance. The multidrug resistance cases are promoting the search for the new alternative methods and pulsed electric field (PEF) treatment could be the alternative or could be used in combination with conventional therapy for the enhancement of the effect. We have shown that nanosecond range PEF is capable to induce apoptosis in the S. cerevisiae as well as in the drug resistant C. lusitaniae and C. guilliermondii. Supplementing the PEF procedure with formic acid (final concentration 0.05%) resulted in improvement of the inactivation efficacy and the induction of apoptosis in the majority of the yeast population. After the treatment yeast were displaying the DNA strand brakes, activation of yeast metacaspase and externalization of phosphatidylserine. Apoptotic phenotypes were registered already after 30 kV/cm × 250 ns × 50 pulses treatment. The highest number of apoptotic yeast cells (>60%) was obtained during the 30 kV/cm × 750 ns × 50 pulses protocol when combined with 0.05% formic acid. The results of our study are useful for development of new non-toxic and effective protocols to induce programed cell death in different yeast species and thus minimize inflammation of the tissue. (Copyright © 2019 Elsevier B.V. All rights reserved.) |
Databáze: | MEDLINE |
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