| Autor: |
Dicker KT, Moore AC, Garabedian NT, Zhang H, Scinto SL, Akins RE; Department of Biomedical Research, A.I. DuPont Hospital for Children , Nemours Children's Clinic , Wilmington , Delaware 19803 , United States., Burris DL, Fox JM, Jia X |
| Jazyk: |
angličtina |
| Zdroj: |
ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2019 May 08; Vol. 11 (18), pp. 16402-16411. Date of Electronic Publication: 2019 Apr 26. |
| DOI: |
10.1021/acsami.9b04383 |
| Abstrakt: |
Fully integrated hydrogel channels were fabricated via interfacial bioorthogonal cross-linking, a diffusion-controlled method for the creation and patterning of synthetic matrices based on the rapid bioorthogonal reaction between s-tetrazines (Tz) and trans-cyclooctene (TCO) dienophiles. Injecting an aqueous solution of a bisTCO cross-linker into a reservoir of tetrazine-modified hyaluronic acid (HA-Tz), while simultaneously drawing the syringe needle through the reservoir, yielded a cross-linked hydrogel channel that was mechanically robust. Fluorescent tags and biochemical signals were spatially patterned into the channel wall through time-dependent perfusion of TCO-conjugated molecules into the lumen of the channel. Different cell populations were spatially encapsulated in the channel wall via temporal alteration of cells in the HA-Tz reservoir. The interfacial approach enabled the spatial patterning of vascular cells, including human abdominal aorta endothelial cells, aortic vascular smooth muscle cells, and aortic adventitial fibroblasts, into the hydrogel channels with high viability and proper morphology in the anatomical order found in human arteries. The bioorthogonal platform does not rely on external triggers and represents the first step toward the engineering of functional and implantable arteries. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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