Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy.

Autor:	Abreu MGL; Universidade Federal Fluminense, Departamento de Odontologia, Área de Periodontia, Nova Friburgo, Rio de Janeiro, Brasil., Kawamoto D; Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia, São Paulo, São Paulo, Brasil., Mayer MPA; Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Microbiologia, São Paulo, São Paulo, Brasil., Pascoal VDB; Universidade Federal Fluminense, Departamento de Ciências Básicas, Nova Friburgo, Rio de Janeiro, Brasil., Caiaffa KS; Universidade Estadual Paulista (UNESP), Faculdade de Odontologia de Araçatuba, Departamento de Endodontia, Araçatuba, São Paulo, Brasil., Zuza EP; Universidade Federal Fluminense, Departamento de Odontologia, Área de Periodontia, Nova Friburgo, Rio de Janeiro, Brasil., Duque C; Universidade Estadual Paulista (UNESP), Faculdade de Odontologia de Araçatuba, Departamento de Odontopediatria e Saúde Pública, Araçatuba, São Paulo, Brasil., Camargo GADCG; Universidade Federal Fluminense, Departamento de Odontologia, Área de Periodontia, Nova Friburgo, Rio de Janeiro, Brasil.
Jazyk:	angličtina
Zdroj:	Journal of applied oral science : revista FOB [J Appl Oral Sci] 2019; Vol. 27, pp. e20180205. Date of Electronic Publication: 2019 Apr 11.
DOI:	10.1590/1678-7757-2018-0205
Abstrakt:	Porphyromonas gingivalis is one of the most important Gram-negative anaerobe bacteria involved in the pathogenesis of periodontitis. P. gingivalis has an arsenal of specialized virulence factors that contribute to its pathogenicity. Among them, fimbriae play a role in the initial attachment and organization of biofilms. Different genotypes of fimA have been related to length of fimbriae and pathogenicity of the bacterium. Objectives: The aim of this study was to identify 5 types of fimA genotype strains in smokers and nonsmokers with periodontitis, before and after periodontal therapy. Material and Methods: Thirty-one patients with periodontitis harboring P. gingivalis were selected: 16 nonsmokers (NS) and 15 smokers (SM). Clinical and microbiological parameters were evaluated at baseline and 3 months after periodontal treatment, namely: plaque index, bleeding on probe, probing depth, gingival recession and clinical attachment level. The frequency of P. gingivalis and fimA genotype strains were determined by polymerase chain reaction. Results: Type I fimA was detected in the majority of SM and NS at baseline, and the frequency did not diminish after 3 months of treatment. The frequency of type II genotype was higher in SM than NS at baseline. After 3 months, statistical reduction was observed only for types II and V fimA genotypes in SM. The highest association was found between types I and II at baseline for NS (37.5%) and SM (53.3%). Conclusion: The most prevalent P. gingivalis fimA genotypes detected in periodontal and smoker patients were genotypes I and II. However, the presence of fimA genotype II was higher in SM. Periodontal treatment was effective in controlling periodontal disease and reducing type II and V P. gingivalis fimA.
Databáze:	MEDLINE
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