Saline extract from Malpighia emarginata DC leaves showed higher polyphenol presence, antioxidant and antifungal activity and promoted cell proliferation in mice splenocytes.

Autor: Barros BRS; Department of Antibiotics, Biosciences Centre, Federal University of Pernambuco, Av. Prof. Artur de Sá, s/n, 50740-520 Recife, PE, Brazil., Barboza BR; Department of Antibiotics, Biosciences Centre, Federal University of Pernambuco, Av. Prof. Artur de Sá, s/n, 50740-520 Recife, PE, Brazil., Ramos BA; Department of Biochemistry, Biosciences Centre, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235, 50670-901 Recife, PE, Brazil., Moura MC; Department of Biochemistry, Biosciences Centre, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235, 50670-901 Recife, PE, Brazil., Coelho LCBB; Department of Biochemistry, Biosciences Centre, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235, 50670-901 Recife, PE, Brazil., Napoleão TH; Department of Biochemistry, Biosciences Centre, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235, 50670-901 Recife, PE, Brazil., Correia MTS; Department of Biochemistry, Biosciences Centre, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235, 50670-901 Recife, PE, Brazil., Paiva PMG; Department of Biochemistry, Biosciences Centre, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235, 50670-901 Recife, PE, Brazil., Cruz Filho IJD; Department of Antibiotics, Biosciences Centre, Federal University of Pernambuco, Av. Prof. Artur de Sá, s/n, 50740-520 Recife, PE, Brazil., Silva TDD; Center for Strategic Technologies of the Northeast, Av. Prof. Luís Freire, 1, 50740-545 Recife, PE, Brazil., Lima CSA; Department of Biophysics, Biosciences Centre, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235, 50670-901 Recife, PE, Brazil., Melo CML; Department of Antibiotics, Biosciences Centre, Federal University of Pernambuco, Av. Prof. Artur de Sá, s/n, 50740-520 Recife, PE, Brazil.
Jazyk: angličtina
Zdroj: Anais da Academia Brasileira de Ciencias [An Acad Bras Cienc] 2019; Vol. 91 (1), pp. e20190916. Date of Electronic Publication: 2019 Apr 08.
DOI: 10.1590/0001-3765201920180358
Abstrakt: Currently, the research of new natural compounds with biological potential demonstrates great ethnopharmacological importance. In this study, we evaluated the biological properties promoted by saline extract from Malpighia emarginata DC leaves, whose objective is to evaluate the antioxidant, antimicrobial and cytotoxicity potential. Phytochemical characterization was performed by UPLC-MS chromatography to identify the chemical compounds. For the antioxidant potential, DPPH, ATT and FRAP methods were used. The antibacterial and antifungal tests were performed evaluating the MIC50, MIC90, CMB and CMF parameters. Moreover, antibiofilm action was evaluated. Cytotoxicity and proliferation were performed using splenocytes from Balb/c mice and were evaluated by cytometry. We found a list of phenolic compounds among other bioactive compounds in the M. emarginata saline extract. In addition, higher antioxidant profile and antifungal activity against different strains of Candida spp. was promoted by the saline extract. Splenocytes showed greater cell viability (more than 90%) and showed higher proliferate index in 24 and 48 hours of incubation with the extract. Saline extract from Malpighia emarginata DC has potential action like antioxidant and antifungal agent without promote animal cell damage.
Databáze: MEDLINE
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