Identification of phenobarbital and other barbiturates in forensic drug screening using positive electrospray ionization liquid chromatography-high resolution mass spectrometry.
Autor: | Høj LJ; Department of Forensic Medicine, University of Copenhagen, Frederik V's vej 11, 3. Floor, 2100, Copenhagen Ø, Denmark., Mollerup CB; Department of Forensic Medicine, University of Copenhagen, Frederik V's vej 11, 3. Floor, 2100, Copenhagen Ø, Denmark., Rasmussen BS; Department of Forensic Medicine, University of Copenhagen, Frederik V's vej 11, 3. Floor, 2100, Copenhagen Ø, Denmark., Johansen SS; Department of Forensic Medicine, University of Copenhagen, Frederik V's vej 11, 3. Floor, 2100, Copenhagen Ø, Denmark., Linnet K; Department of Forensic Medicine, University of Copenhagen, Frederik V's vej 11, 3. Floor, 2100, Copenhagen Ø, Denmark., Dalsgaard PW; Department of Forensic Medicine, University of Copenhagen, Frederik V's vej 11, 3. Floor, 2100, Copenhagen Ø, Denmark. |
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Jazyk: | angličtina |
Zdroj: | Drug testing and analysis [Drug Test Anal] 2019 Aug; Vol. 11 (8), pp. 1258-1263. Date of Electronic Publication: 2019 May 24. |
DOI: | 10.1002/dta.2603 |
Abstrakt: | Comprehensive drug-screening performed by liquid chromatography-high resolution mass spectrometry (LC-HRMS) enables identification of hundreds to thousands of drug compounds in a single analysis. Forensic drug screening is generally performed with positive electrospray ionization (ESI + ), targeting basic drugs; however, a few toxicologically important drugs such as barbiturates, may require analysis by negative ESI. In this work, screening targets for barbiturates were determined using our LC-HRMS screening with ESI + . For several years, our forensic whole blood samples have been analyzed using the LC-HRMS-ESI + screening in parallel with a multi-target LC-MS/MS-ESI - method. From 2014 to 2018, 23 samples were positive for phenobarbital (0.5-81 mg/kg). Retrospective data analysis of 4816 blood samples (15 positive) revealed several potential screening targets for phenobarbital. The targets were tentatively identified by exact mass and isotopic pattern as uncommon adducts of phenobarbital and as a decomposition product of phenobarbital N-glucoside (C (© 2019 John Wiley & Sons, Ltd.) |
Databáze: | MEDLINE |
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