| Autor: |
Paun G; National Institute for Research-Development of Biological Sciences, Bucharest 6, 060031, Romania., Neagu E; National Institute for Research-Development of Biological Sciences, Bucharest 6, 060031, Romania., Moroeanu V; National Institute for Research-Development of Biological Sciences, Bucharest 6, 060031, Romania., Albu C; National Institute for Research-Development of Biological Sciences, Bucharest 6, 060031, Romania., Savin S; National Institute for Research-Development of Biological Sciences, Bucharest 6, 060031, Romania., Lucian Radu G; National Institute for Research-Development of Biological Sciences, Bucharest 6, 060031, Romania.; Faculty of Applied Chemistry and Materials Science, Politehnica University of Bucharest, Bucharest, Romania. |
| Abstrakt: |
This study evaluated the biological activities of Eryngium planum and of Cnicus benedictus extracts enriched in polyphenols obtained by nanofiltration. The HPLC-MS analysis showed that E. planum contains mainly flavonoids, especially rutin, while in C. benedictus extracts show the high concentration of the phenolic acids, principally the chlorogenic acid and sinapic acid. Herein, there is the first report of ursolic acid, genistin, and isorhamnetin in E. planum and C. benedictus. C. benedictus polyphenolic-rich extract showed high scavenging activity (IC 50 =0.0081 mg/mL) comparable to that of standard compound (ascorbic acid) and a higher reducing power (IC 50 = 0.082 mg/mL), with IC 50 having a significantly lower value than IC 50 for ascorbic acid. Both extracts were nontoxic to NCTC cell line. Among the investigated herbs, E. planum polyphenolic-rich extract showed the highest inhibitory activities with the IC 50 value of 31.3 μ g/mL for lipoxygenase and 24.6 μ g/mL for hyaluronidase. Both polyphenolic-rich extracts had a higher inhibitory effect on α -amylase and α -glucosidase than that of the acarbose. The synergistic effect of ursolic acid, rutin, chlorogenic acid, rosmarinic acid, genistin, and daidzein identified in polyphenolic-rich extracts could be mainly responsible for the pharmacological potentials of the studied extracts used in managing inflammation and diabetes. |
