Effects of Tributyltin (TBT) on Rat Bone and Mineral Metabolism.

Autor:	Resgala LCR; Aging Cell Biology Laboratory, Department of Morphology, UFES, Vitória, Brazil.; Programa de Pós Graduação em Biotecnologia (UFES/RENORBIO), Health Sciences Center, UFES, Vitória, Brazil.; Centro Universitário Redentor (UniREDENTOR), Itaperuna, Brazil., Santana HS; Aging Cell Biology Laboratory, Department of Morphology, UFES, Vitória, Brazil.; Programa de Pós Graduação em Bioquímica e Farmacologia, Department of Physiological Sciences, Health Sciences Center, UFES, Vitória, Brazil., Portela BSM; Aging Cell Biology Laboratory, Department of Morphology, UFES, Vitória, Brazil.; Centro Universitário Redentor (UniREDENTOR), Itaperuna, Brazil.; Hospital Universitário Antônio Cassiano de Moraes (HUCAM), Health Sciences Center, UFES, Vitória, Brazil., Zanovello MVS; Aging Cell Biology Laboratory, Department of Morphology, UFES, Vitória, Brazil., da Costa CS; Programa de Pós Graduação em Ciências Fisiológicas, Department of Physiological Sciences, Health Sciences Center, UFES, Vitória, Brazil.; Laboratory of Endocrinology and Cellular Toxicology, Department of Morphology, Health Sciences Center, UFES, Vitória, Brazil., Niño OMS; Programa de Pós Graduação em Ciências Fisiológicas, Department of Physiological Sciences, Health Sciences Center, UFES, Vitória, Brazil.; Laboratory of Endocrinology and Cellular Toxicology, Department of Morphology, Health Sciences Center, UFES, Vitória, Brazil., Endlich Bicudo N; Aging Cell Biology Laboratory, Department of Morphology, UFES, Vitória, Brazil., Dos Santos DZ; Laboratory of Cellular and Molecular Biology of Human Cancer, Department of Pharmaceutical Sciences, Health Sciences Center, UFES, Vitória, Brazil., Lang Podratz P; Programa de Pós Graduação em Ciências Fisiológicas, Department of Physiological Sciences, Health Sciences Center, UFES, Vitória, Brazil.; Laboratory of Endocrinology and Cellular Toxicology, Department of Morphology, Health Sciences Center, UFES, Vitória, Brazil., da Cunha M; Laboratory of Cell Biology, Biotechnology Center, UENF, Campos dos Goytacazes, Vitória, Brazil., Oliveira ALA; Laboratory of Cell Biology, Biotechnology Center, UENF, Campos dos Goytacazes, Vitória, Brazil., Ceotto Freitas Lima L; Programa de Pós Graduação em Ciências Fisiológicas, Department of Physiological Sciences, Health Sciences Center, UFES, Vitória, Brazil.; Laboratory of Endocrinology and Cellular Toxicology, Department of Morphology, Health Sciences Center, UFES, Vitória, Brazil., Gomes-Rochette NF; Aging Cell Biology Laboratory, Department of Morphology, UFES, Vitória, Brazil., Rangel LBA; Programa de Pós Graduação em Biotecnologia (UFES/RENORBIO), Health Sciences Center, UFES, Vitória, Brazil.; Programa de Pós Graduação em Bioquímica e Farmacologia, Department of Physiological Sciences, Health Sciences Center, UFES, Vitória, Brazil.; Laboratory of Cellular and Molecular Biology of Human Cancer, Department of Pharmaceutical Sciences, Health Sciences Center, UFES, Vitória, Brazil., Graceli JB; Programa de Pós Graduação em Ciências Fisiológicas, Department of Physiological Sciences, Health Sciences Center, UFES, Vitória, Brazil.; Laboratory of Endocrinology and Cellular Toxicology, Department of Morphology, Health Sciences Center, UFES, Vitória, Brazil, jbgraceli@gmail.com., Silva IV; Aging Cell Biology Laboratory, Department of Morphology, UFES, Vitória, Brazil.; Programa de Pós Graduação em Biotecnologia (UFES/RENORBIO), Health Sciences Center, UFES, Vitória, Brazil.; Programa de Pós Graduação em Bioquímica e Farmacologia, Department of Physiological Sciences, Health Sciences Center, UFES, Vitória, Brazil, lbce18@yahoo.com.
Jazyk:	angličtina
Zdroj:	Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology [Cell Physiol Biochem] 2019; Vol. 52 (5), pp. 1166-1177.
DOI:	10.33594/000000079
Abstrakt:	Background/aims: Tributyltin (TBT) is an organotin (OTs) and biohazard organometallic pollutant. Recently our group has shown that TBT, even in very low doses, has deleterious effects on several tissues most likely due to its role as an endocrine-disrupting molecule. Other studies have confirmed that OT exposure could be responsible for neural, endocrine, and reproductive dysfunctions via in vitro and in vivo models. However, TBT effects on bone lack concise data despite the fact that bone turnover is regulated by endocrine molecules, such as parathormone (PTH), estrogen (E2), etc. Our group has already shown that TBT disrupts adrenal and female gonadal functions. Methods: We studied the effects of TBT on bone metabolism and structure using DXA, microCT scan, and SEM. We also determined the calcium (Ca²⁺) and phosphate (Pi) metabolism in TBT-treated rats as well as some biomarkers for bone formation and resorption. Results: Surprisingly, we found that TBT leads to higher bone mineral density (BMD) although lesions in spinal bone were observed by either microCT scan or SEM. Biomarkers for bone resorption, such as the urinary deoxipyridinolines (DPD) excretion ratio was increased in TBT-treated animals versus mock-treated controls. Osteocalcin (OC) and alkaline phosphatase (AP) are markers of bone formation and are also elevated suggesting that the bone matrix suffers from a higher turnover. Serum Ca²⁺ (total and ionized) do not changed by TBT treatment although hypercalciuria is observed. Conclusion: It is known that Sn atoms have three valence states (Sn²⁺, Sn³⁺, and Sn⁴⁺); hence, we hypothesized that Sn (more likely Sn²⁺) could be competing with Ca²⁺ and/or Mg²⁺ in hydroxyapatite mineral matrix to disturb bone turnover. Further work is needed to confirm this hypothesis. Competing Interests: The authors declare that they have no conflicts of interest. (© Copyright by the Author(s). Published by Cell Physiol Biochem Press.)
Databáze:	MEDLINE
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