Further characterisation of Leucocytozoon podargii in wild tawny frogmouths (Podargus strigoides) in Western Australia.

Autor: Jiang Y; School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia, 6150, Australia.; Jiangsu Academy of Agriculture Sciences, Nanjing, 210014, China., Brice B; Kanyana Wildlife Rehabilitation Centre, 120 Gilchrist Road, Lesmurdie, Western Australia, 6076, Australia., Nguyen M; Sustainability and Biosecurity, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, Western Australia, 6151, Australia., Loh R; Sustainability and Biosecurity, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, Western Australia, 6151, Australia., Greay T; School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia, 6150, Australia., Adlard R; Queensland Museum Network, South Brisbane, Queensland, 4101, Australia., Ryan U; School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia, 6150, Australia., Yang R; School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia, 6150, Australia. R.Yang@murdoch.edu.au.
Jazyk: angličtina
Zdroj: Parasitology research [Parasitol Res] 2019 Jun; Vol. 118 (6), pp. 1833-1840. Date of Electronic Publication: 2019 Apr 15.
DOI: 10.1007/s00436-019-06317-7
Abstrakt: The present study assessed the prevalence and morphology of Leucocytozoon podargii from wild tawny frogmouths (Podargus strigoides) in Western Australia (WA) and genetically characterised the cytochrome b gene (cyt b) of L. podargii in wild tawny frogmouths from WA and Queensland (QLD). The prevalence of L. podargii in wild tawny frogmouths from WA was 93.3% (14/15; 95% CI, 68.1-99.8%). The morphological characters of L. podargii from WA were similar to L. podargii from QLD: the gametocytes were round-oval shape, approximately 8-12 μm in diameter; the macrogametocytes were 12.4 μm in diameter; microgametocytes were 10.4 μm in diameter; and the ratio of macrogametocytes and microgametocytes was 3:2. Sequence analysis of partial cyt b gene fragments revealed that L. podargii sequences isolated from wild tawny frogmouths in WA shared the highest similarity (99.8% at nucleotide level and 100% at protein level) with L. podargii isolated from wild tawny frogmouths in QLD. The mitochondrial 18S rRNA gene of L. podargii gametocytes was quantified using droplet digital PCR (ddPCR), and the highest gametocyte load was detected in the lung. This finding corresponds to the results of the histological study. Based on the morphological and molecular studies, it was concluded that the Leucocytozoon parasite identified from wild tawny frogmouths in WA is consistent with L. podargii from wild tawny frogmouths in QLD, and the present study has genetically characterised two different L. podargii genotypes (QLD and WA) for the first time.
Databáze: MEDLINE
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