A Bright Future for Fluorescence Imaging of Fungi in Living Hosts.
| Autor: | Chapuis AF; MRC Centre for Medical Mycology at the University of Aberdeen, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK. r01afc16@abdn.ac.uk., Ballou ER; Institute of Microbiology and Infection, School of Biosciences, University of Birmingham, Birmingham B15 2TT, UK. E.R.Ballou@bham.ac.uk., MacCallum DM; MRC Centre for Medical Mycology at the University of Aberdeen, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, UK. d.m.maccallum@abdn.ac.uk. |
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| Jazyk: | angličtina |
| Zdroj: | Journal of fungi (Basel, Switzerland) [J Fungi (Basel)] 2019 Apr 03; Vol. 5 (2). Date of Electronic Publication: 2019 Apr 03. |
| DOI: | 10.3390/jof5020029 |
| Abstrakt: | Traditional in vivo investigation of fungal infection and new antifungal therapies in mouse models is usually carried out using post mortem methodologies. However, biomedical imaging techniques focusing on non-invasive techniques using bioluminescent and fluorescent proteins have become valuable tools. These new techniques address ethical concerns as they allow reduction in the number of animals required to evaluate new antifungal therapies. They also allow better understanding of the growth and spread of the pathogen during infection. In this review, we concentrate on imaging technologies using different fungal reporter proteins. We discuss the advantages and limitations of these different reporters and compare the efficacy of bioluminescent and fluorescent proteins for fungal research. |
| Databáze: | MEDLINE |
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