Comparison of cell mechanical measurements provided by Atomic Force Microscopy (AFM) and Micropipette Aspiration (MPA).

Autor: Daza R; Centro de Tecnología Biomédica, Universidad Politécnica de Madrid, 28223, Pozuelo de Alarcón, Madrid, Spain; Departamento de Ciencia de Materiales, ETSI Caminos, Canales y Puertos, Universidad Politécnica de Madrid, 28040, Madrid, Spain. Electronic address: rafael.daza@upm.es., González-Bermúdez B; Centro de Tecnología Biomédica, Universidad Politécnica de Madrid, 28223, Pozuelo de Alarcón, Madrid, Spain; Departamento de Ciencia de Materiales, ETSI Caminos, Canales y Puertos, Universidad Politécnica de Madrid, 28040, Madrid, Spain., Cruces J; Departamento de Fisiología Animal II, Facultad de Biología, Universidad Complutense de Madrid, 28040, Madrid, Spain., De la Fuente M; Departamento de Fisiología Animal II, Facultad de Biología, Universidad Complutense de Madrid, 28040, Madrid, Spain., Plaza GR; Centro de Tecnología Biomédica, Universidad Politécnica de Madrid, 28223, Pozuelo de Alarcón, Madrid, Spain; Departamento de Ciencia de Materiales, ETSI Caminos, Canales y Puertos, Universidad Politécnica de Madrid, 28040, Madrid, Spain., Arroyo-Hernández M; Centro de Tecnología Biomédica, Universidad Politécnica de Madrid, 28223, Pozuelo de Alarcón, Madrid, Spain; Departamento de Biotecnología, Faculty of Experimental Sciences, Francisco de Vitoria University (UFV), 28223, Pozuelo de Alarcón, Madrid, Spain., Elices M; Centro de Tecnología Biomédica, Universidad Politécnica de Madrid, 28223, Pozuelo de Alarcón, Madrid, Spain; Departamento de Ciencia de Materiales, ETSI Caminos, Canales y Puertos, Universidad Politécnica de Madrid, 28040, Madrid, Spain., Pérez-Rigueiro J; Centro de Tecnología Biomédica, Universidad Politécnica de Madrid, 28223, Pozuelo de Alarcón, Madrid, Spain; Departamento de Ciencia de Materiales, ETSI Caminos, Canales y Puertos, Universidad Politécnica de Madrid, 28040, Madrid, Spain; CIBER-BBN, Biomedical Research Networking Center in Bioengineering Biomaterials and Nanomedicine, Spain., Guinea GV; Centro de Tecnología Biomédica, Universidad Politécnica de Madrid, 28223, Pozuelo de Alarcón, Madrid, Spain; Departamento de Ciencia de Materiales, ETSI Caminos, Canales y Puertos, Universidad Politécnica de Madrid, 28040, Madrid, Spain; CIBER-BBN, Biomedical Research Networking Center in Bioengineering Biomaterials and Nanomedicine, Spain.
Jazyk: angličtina
Zdroj: Journal of the mechanical behavior of biomedical materials [J Mech Behav Biomed Mater] 2019 Jul; Vol. 95, pp. 103-115. Date of Electronic Publication: 2019 Apr 08.
DOI: 10.1016/j.jmbbm.2019.03.031
Abstrakt: A comparative analysis of T-lymphocyte mechanical data obtained from Micropipette Aspiration (MPA) and Atomic Force Microscopy (AFM) is presented. Results obtained by fitting the experimental data to simple Hertz and Theret models led to non-Gaussian distributions and significantly different values of the elastic moduli obtained by both techniques. The use of more refined models, taking into account the finite size of cells (simplified double contact and Zhou models) reduces the differences in the values calculated for the elastic moduli. Several possible sources for the discrepancy between the techniques are considered. The analysis suggests that the local nature of AFM measurements compared with the more general character of MPA measurements probably contributed to the differences observed.
(Copyright © 2019 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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