Metagenomic 16S rRNA gene sequencing survey of Borrelia species in Irish samples of Ixodes ricinus ticks.

Autor: Lambert JS; University College Dublin, Dublin, Ireland.; Mater Misericordiae University Hospital, Dublin, Ireland., Cook MJ; Vis a Vis Symposiums, Highcliffe, Dorset, United Kingdom., Healy JE; University College Cork, Cork, Ireland., Murtagh R; University College Dublin, Dublin, Ireland., Avramovic G; University College Dublin, Dublin, Ireland.; Mater Misericordiae University Hospital, Dublin, Ireland., Lee SH; Milford Molecular Diagnostics, Connecticut, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2019 Apr 15; Vol. 14 (4), pp. e0209881. Date of Electronic Publication: 2019 Apr 15 (Print Publication: 2019).
DOI: 10.1371/journal.pone.0209881
Abstrakt: The spirochetal bacterium Borrelia miyamotoi is a human pathogen and has been identified in many countries throughout the world. This study reports for the first time the presence of Borrelia miyamotoi in Ireland, and confirms prior work with the detection of B. garinii and B. valaisiana infected ticks. Questing Ixodes ricinus nymph samples were taken at six localities within Ireland. DNA extraction followed by Sanger sequencing was used to identify the species and strains present in each tick. The overall rate of borrelial infection in the Irish tick population was 5%, with a range from 2% to 12% depending on the locations of tick collection. The most prevalent species detected was B. garinii (70%) followed by B. valaisiana (20%) and B. miyamotoi (10%). Knowledge of Borrelia species prevalence is important and will guide appropriate selection of antigens for serology test kit manufacture, help define the risk of infection, and allow medical authorities to formulate appropriate strategies and guidelines for diagnosis and treatment of Borrelia diseases.
Competing Interests: SHL is Director of Milford Molecular Diagnostics Laboratory. There are no patents, products in development or marketed products associated with this research to declare. This does not alter our adherence to PLOS ONE policies on sharing data and materials.
Databáze: MEDLINE
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