Strategy for Structural Elucidation of Polysaccharides: Elucidation of a Maize Mucilage that Harbors Diazotrophic Bacteria.

Autor:	Amicucci MJ; Department of Chemistry , University of California-Davis , Davis , California 95616 , United States., Galermo AG; Department of Chemistry , University of California-Davis , Davis , California 95616 , United States., Guerrero A; Department of Chemistry , University of California-Davis , Davis , California 95616 , United States., Treves G; Department of Chemistry , University of California-Davis , Davis , California 95616 , United States., Nandita E; Department of Chemistry , University of California-Davis , Davis , California 95616 , United States., Kailemia MJ; Department of Chemistry , University of California-Davis , Davis , California 95616 , United States., Higdon SM; Department of Plant Sciences , University of California-Davis , Davis , California 95616 , United States., Pozzo T; Department of Plant Sciences , University of California-Davis , Davis , California 95616 , United States., Labavitch JM; Department of Plant Sciences , University of California-Davis , Davis , California 95616 , United States., Bennett AB; Department of Plant Sciences , University of California-Davis , Davis , California 95616 , United States., Lebrilla CB; Department of Chemistry , University of California-Davis , Davis , California 95616 , United States.
Jazyk:	angličtina
Zdroj:	Analytical chemistry [Anal Chem] 2019 Jun 04; Vol. 91 (11), pp. 7254-7265. Date of Electronic Publication: 2019 Apr 30.
DOI:	10.1021/acs.analchem.9b00789
Abstrakt:	The recruitment of a bacterial consortium by the host is a strategy not limited to animals but is also used in plants. A maize aerial root mucilage has been found that harbors nitrogen fixing bacteria that are attracted to the carbohydrate rich environment. This synbiotic relationship is facilitated by a polysaccharide, whose complicated structure has been previously unknown. In this report, we present the characterization of the maize polysaccharide by employing new analytical strategies combining chemical depolymerization, oligosaccharide sequencing, and monosaccharide and glycosidic linkage quantitation. The mucilage contains a single heterogeneous polysaccharide composed of a highly fucosylated and xylosylated galactose backbone with arabinan and mannoglucuronan branches. This unique polysaccharide structure may select for the diazotrophic community by containing monosaccharides and linkages that correspond to the glycosyl hydrolases associated with the microbial community. The elucidation of this complicated structure illustrates the power of the analytical methods, which may serve as a general platform for polysaccharide analysis in the future.
Databáze:	MEDLINE
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