Active Transport of Membrane Components by Self-Organization of the Min Proteins.

Autor:	Shih YL; Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan. Electronic address: ylshih10@gate.sinica.edu.tw., Huang LT; Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan., Tu YM; Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan., Lee BF; Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan., Bau YC; Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan., Hong CY; Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan., Lee HL; Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan., Shih YP; Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan., Hsu MF; Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan., Lu ZX; Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan., Chen JS; Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan., Chao L; Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan. Electronic address: lingchao@ntu.edu.tw.
Jazyk:	angličtina
Zdroj:	Biophysical journal [Biophys J] 2019 Apr 23; Vol. 116 (8), pp. 1469-1482. Date of Electronic Publication: 2019 Mar 23.
DOI:	10.1016/j.bpj.2019.03.011
Abstrakt:	Heterogeneous distribution of components in the biological membrane is critical in the process of cell polarization. However, little is known about the mechanisms that can generate and maintain the heterogeneous distribution of the membrane components. Here, we report that the propagating wave patterns of the bacterial Min proteins can impose steric pressure on the membrane, resulting in transport and directional accumulation of the component in the membrane. Therefore, the membrane component waves represent transport of the component in the membrane that is caused by the steric pressure gradient induced by the differential levels of binding and dissociation of the Min proteins in the propagating waves on the membrane surface. The diffusivity, majorly influenced by the membrane anchor of the component, and the repulsed ability, majorly influenced by the steric property of the membrane component, determine the differential spatial distribution of the membrane component. Thus, transportation of the membrane component by the Min proteins follows a simple physical principle, which resembles a linear peristaltic pumping process, to selectively segregate and maintain heterogeneous distribution of materials in the membrane. VIDEO ABSTRACT. (Copyright © 2019 Biophysical Society. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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