| Autor: |
de Haro-Cruz MJ; Department of Microbiology, Escuela Nacional de Ciencias Biológicas del Instituto Politécnico Nacional, Carpio y Pro. Plan de Ayala, Col. Casco de Sto. Thomas, C.P. 11340, Mexico City, México., Guadarrama-Macedo SI; Virology Laboratory, National Institute of Perinatology, Isidro Espinoza de los Reyes, Montes Urales 800, Col. Lomas Virreyes, CP 11000, Mexico City, Mexico., López-Hurtado M; Virology Laboratory, National Institute of Perinatology, Isidro Espinoza de los Reyes, Montes Urales 800, Col. Lomas Virreyes, CP 11000, Mexico City, Mexico., Escobedo-Guerra MR; Virology Laboratory, National Institute of Perinatology, Isidro Espinoza de los Reyes, Montes Urales 800, Col. Lomas Virreyes, CP 11000, Mexico City, Mexico., Guerra-Infante FM; Department of Microbiology, Escuela Nacional de Ciencias Biológicas del Instituto Politécnico Nacional, Carpio y Pro. Plan de Ayala, Col. Casco de Sto. Thomas, C.P. 11340, Mexico City, México. fguerra_96@yahoo.com.; Virology Laboratory, National Institute of Perinatology, Isidro Espinoza de los Reyes, Montes Urales 800, Col. Lomas Virreyes, CP 11000, Mexico City, Mexico. fguerra_96@yahoo.com. |
| Abstrakt: |
Chlamydia trachomatis is considered as a public health problem due to its high prevalence and increased rates of gynecological disorders. The major outer membrane protein (MOMP) of this bacterium is the most abundant protein in its membrane and has been evaluated not only as a vaccine development candidate but also is used in many diagnostic tests. The MOMP weighs 69 kDa and contains four variable segments (VS 1-4) separated by constant regions. Several research groups have developed recombinant single-variable segments of MOMP expressed in Escherichia coli cytoplasm. But, all variable segments have been used minimally for the diagnosis of a chlamydial infection. In this experiment, the authors obtained the recombinant MOMP of C. trachomatis (rMOMP) in E. coli rMOMP and extracted, purified, and partially characterized it. This was later used to identify anti-Chlamydia trachomatis antibodies in sera of infertile patients by immunodetection assays, enzyme-linked immunosorbent assay (ELISA), and indirect immunofluorescence tests. The ELISA test showed high sensitivity and low specificity of 100 and 58.3%, respectively. The above results obtained were linked to the cross-reactivity of antibodies against C. pneumoniae or C. psittaci. Hence, an evaluation was performed to obtain an optimized test for the diagnosis of C. trachomatis infection. |
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