Tracking of enzymatic biomass deconstruction by fungal secretomes highlights markers of lignocellulose recalcitrance.
Autor: | Paës G; 1FARE Laboratory, INRA, Université de Reims Champagne-Ardenne, Reims, France., Navarro D; 2INRA, Aix Marseille Univ., UMR1163, BBF, Biodiversité et Biotechnologie Fongiques, Marseille, France.; 3INRA, Aix-Marseille Univ., UMR1163, CIRM-CF, Marseille, France., Benoit Y; 4IFP Energies Nouvelles, Rueil-Malmaison, France., Blanquet S; 4IFP Energies Nouvelles, Rueil-Malmaison, France., Chabbert B; 1FARE Laboratory, INRA, Université de Reims Champagne-Ardenne, Reims, France., Chaussepied B; 4IFP Energies Nouvelles, Rueil-Malmaison, France., Coutinho PM; 5CNRS, Aix-Marseille Univ., UMR7857 AFMB, Architecture et Fonction des Macromolécules Biologiques, Marseille, France., Durand S; 6INRA, UR1268 Biopolymères Interactions Assemblages, Nantes, France., Grigoriev IV; 7US Department of Energy Joint Genome Institute, Walnut Creek, CA USA.; 8Department of Plant and Microbial Biology, University of California Berkeley, Berkeley, CA USA., Haon M; 2INRA, Aix Marseille Univ., UMR1163, BBF, Biodiversité et Biotechnologie Fongiques, Marseille, France., Heux L; 9CNRS, Univ. Grenoble Alpes, CERMAV, Grenoble, France., Launay C; 6INRA, UR1268 Biopolymères Interactions Assemblages, Nantes, France., Margeot A; 4IFP Energies Nouvelles, Rueil-Malmaison, France., Nishiyama Y; 9CNRS, Univ. Grenoble Alpes, CERMAV, Grenoble, France., Raouche S; 2INRA, Aix Marseille Univ., UMR1163, BBF, Biodiversité et Biotechnologie Fongiques, Marseille, France., Rosso MN; 2INRA, Aix Marseille Univ., UMR1163, BBF, Biodiversité et Biotechnologie Fongiques, Marseille, France., Bonnin E; 6INRA, UR1268 Biopolymères Interactions Assemblages, Nantes, France., Berrin JG; 2INRA, Aix Marseille Univ., UMR1163, BBF, Biodiversité et Biotechnologie Fongiques, Marseille, France. |
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Jazyk: | angličtina |
Zdroj: | Biotechnology for biofuels [Biotechnol Biofuels] 2019 Apr 01; Vol. 12, pp. 76. Date of Electronic Publication: 2019 Apr 01 (Print Publication: 2019). |
DOI: | 10.1186/s13068-019-1417-8 |
Abstrakt: | Background: Lignocellulose biomass is known as a recalcitrant material towards enzymatic hydrolysis, increasing the process cost in biorefinery. In nature, filamentous fungi naturally degrade lignocellulose, using an arsenal of hydrolytic and oxidative enzymes. Assessment of enzyme hydrolysis efficiency generally relies on the yield of glucose for a given biomass. To better understand the markers governing recalcitrance to enzymatic degradation, there is a need to enlarge the set of parameters followed during deconstruction. Results: Industrially-pretreated biomass feedstocks from wheat straw, miscanthus and poplar were sequentially hydrolysed following two steps. First, standard secretome from Trichoderma reesei was used to maximize cellulose hydrolysis, producing three recalcitrant lignin-enriched solid substrates. Then fungal secretomes from three basidiomycete saprotrophs ( Laetisaria arvalis, Artolenzites elegans and Trametes ljubarskyi ) displaying various hydrolytic and oxidative enzymatic profiles were applied to these recalcitrant substrates, and compared to the T. reesei secretome. As a result, most of the glucose was released after the first hydrolysis step. After the second hydrolysis step, half of the remaining glucose amount was released. Overall, glucose yield after the two sequential hydrolyses was more dependent on the biomass source than on the fungal secretomes enzymatic profile. Solid residues obtained after the two hydrolysis steps were characterized using complementary methodologies. Correlation analysis of several physico-chemical parameters showed that released glucose yield was negatively correlated with lignin content and cellulose crystallinity while positively correlated with xylose content and water sorption. Water sorption appears as a pivotal marker of the recalcitrance as it reflects chemical and structural properties of lignocellulosic biomass. Conclusions: Fungal secretomes applied to highly recalcitrant biomass samples can further extend the release of the remaining glucose. The glucose yield can be correlated to chemical and physical markers, which appear to be independent from the biomass type and secretome. Overall, correlations between these markers reveal how nano-scale properties (polymer content and organization) influence macro-scale properties (particle size and water sorption). Further systematic assessment of these markers during enzymatic degradation will foster the development of novel cocktails to unlock the degradation of lignocellulose biomass. Competing Interests: The authors declare that they have no competing interests. |
Databáze: | MEDLINE |
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