The natural history of Get3-like chaperones.

Autor: Farkas Á; Department of Molecular Biology, Göttingen University Medical Center, Göttingen, Germany., De Laurentiis EI; Department of Molecular Biology, Göttingen University Medical Center, Göttingen, Germany., Schwappach B; Department of Molecular Biology, Göttingen University Medical Center, Göttingen, Germany.; Max-Planck Institute for Biophysical Chemistry, Göttingen, Germany.
Jazyk: angličtina
Zdroj: Traffic (Copenhagen, Denmark) [Traffic] 2019 May; Vol. 20 (5), pp. 311-324.
DOI: 10.1111/tra.12643
Abstrakt: Get3 in yeast or TRC40 in mammals is an ATPase that, in eukaryotes, is a central element of the GET or TRC pathway involved in the targeting of tail-anchored proteins. Get3 has also been shown to possess chaperone holdase activity. A bioinformatic assessment was performed across all domains of life on functionally important regions of Get3 including the TRC40-insert and the hydrophobic groove essential for tail-anchored protein binding. We find that such a hydrophobic groove is much more common in bacterial Get3 homologs than previously appreciated based on a directed comparison of bacterial ArsA and yeast Get3. Furthermore, our analysis shows that the region containing the TRC40-insert varies in length and methionine content to an unexpected extent within eukaryotes and also between different phylogenetic groups. In fact, since the TRC40-insert is present in all domains of life, we suggest that its presence does not automatically predict a tail-anchored protein targeting function. This opens up a new perspective on the function of organellar Get3 homologs in plants which feature the TRC40-insert but have not been demonstrated to function in tail-anchored protein targeting. Our analysis also highlights a large diversity of the ways Get3 homologs dimerize. Thus, based on the structural features of Get3 homologs, these proteins may have an unexplored functional diversity in all domains of life.
(© 2019 The Authors. Traffic published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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