A novel role for the late-onset Alzheimer's disease (LOAD)-associated protein Bin1 in regulating postsynaptic trafficking and glutamatergic signaling.

Autor: Schürmann B; Department of Physiology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.; Department of Molecular Psychiatry, University of Bonn, Bonn, Germany., Bermingham DP; Department of Physiology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA., Kopeikina KJ; Department of Physiology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA., Myczek K; Department of Physiology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA., Yoon S; Department of Physiology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA., Horan KE; Department of Physiology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA., Kelly CJ; Department of Physiology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA., Martin-de-Saavedra MD; Department of Physiology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA., Forrest MP; Department of Physiology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA., Fawcett-Patel JM; Department of Physiology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA., Smith KR; Department of Physiology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA., Gao R; Department of Physiology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA., Bach A; Department of Physiology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA., Burette AC; University of North Carolina, Chapel Hill, NC, USA., Rappoport JZ; Center for Advanced Microscopy and Nikon Imaging Center, Northwestern University Feinberg School of Medicine, Chicago, IL, USA., Weinberg RJ; University of North Carolina, Chapel Hill, NC, USA., Martina M; Department of Physiology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA., Penzes P; Department of Physiology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA. p-penzes@northwestern.edu.; Department of Psychiatry and Behavioral Sciences, Northwestern University Feinberg School of Medicine, Chicago, IL, USA. p-penzes@northwestern.edu.
Jazyk: angličtina
Zdroj: Molecular psychiatry [Mol Psychiatry] 2020 Sep; Vol. 25 (9), pp. 2000-2016. Date of Electronic Publication: 2019 Apr 09.
DOI: 10.1038/s41380-019-0407-3
Abstrakt: Postsynaptic trafficking plays a key role in regulating synapse structure and function. While spiny excitatory synapses can be stable throughout adult life, their morphology and function is impaired in Alzheimer's disease (AD). However, little is known about how AD risk genes impact synaptic function. Here we used structured superresolution illumination microscopy (SIM) to study the late-onset Alzheimer's disease (LOAD) risk factor BIN1, and show that this protein is abundant in postsynaptic compartments, including spines. While postsynaptic Bin1 shows colocalization with clathrin, a major endocytic protein, it also colocalizes with the small GTPases Rab11 and Arf6, components of the exocytic pathway. Bin1 participates in protein complexes with Arf6 and GluA1, and manipulations of Bin1 lead to changes in spine morphology, AMPA receptor surface expression and trafficking, and AMPA receptor-mediated synaptic transmission. Our data provide new insights into the mesoscale architecture of postsynaptic trafficking compartments and their regulation by a major LOAD risk factor.
Databáze: MEDLINE
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