| Autor: |
Pelegrino MT; Center for Natural and Human Sciences, Universidade Federal do ABC, Av. dos Estados 5001, Santo André, SP, CEP 09210-580, Brazil. pelegrino.milena@ufabc.edu.br.; Nanomedicine Research Unit (NANOMED), Universidade Federal do ABC, Av. dos Estados 5001, Santo André, SP 09210-580, Brazil. pelegrino.milena@ufabc.edu.br., De Araujo Lima B; Tropical Disease Laboratory, Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas, Campinas, SP 13083-862, Brazil. brunalimabio@gmail.com., Do Nascimento MHM; Nanomedicine Research Unit (NANOMED), Universidade Federal do ABC, Av. dos Estados 5001, Santo André, SP 09210-580, Brazil. monica.nasci@hotmail.com.; Tropical Disease Laboratory, Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas, Campinas, SP 13083-862, Brazil. monica.nasci@hotmail.com., Lombello CB; Center for Natural and Human Sciences, Universidade Federal do ABC, Av. dos Estados 5001, Santo André, SP, CEP 09210-580, Brazil. Christiane.lombello@ufabc.edu.br.; Center for Engineering, Modeling and Applied Social Science, Universidade Federal do ABC, Alameda da Universidade sem numero, São Bernardo do Campo, SP, CEP 09606-045, Brazil. Christiane.lombello@ufabc.edu.br., Brocchi M; Tropical Disease Laboratory, Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas, Campinas, SP 13083-862, Brazil. mbrocchi@unicamp.br., Seabra AB; Center for Natural and Human Sciences, Universidade Federal do ABC, Av. dos Estados 5001, Santo André, SP, CEP 09210-580, Brazil. amedea.seabra@ufabc.edu.br.; Nanomedicine Research Unit (NANOMED), Universidade Federal do ABC, Av. dos Estados 5001, Santo André, SP 09210-580, Brazil. amedea.seabra@ufabc.edu.br. |
| Abstrakt: |
Nitric oxide (NO) is involved in physiological processes, including vasodilatation, wound healing and antibacterial activities. As NO is a free radical, designing drugs to generate therapeutic amounts of NO in controlled spatial and time manners is still a challenge. In this study, the NO donor S -nitrosoglutathione (GSNO) was incorporated into the thermoresponsive Pluronic F-127 (PL)-chitosan (CS) hydrogel, with an easy and economically feasible methodology. CS is a polysaccharide with known antimicrobial properties. Scanning electron microscopy, rheology and differential scanning calorimetry techniques were used for hydrogel characterization. The results demonstrated that the hydrogel has a smooth surface, thermoresponsive behavior and good mechanical stability. The kinetics of NO release and GSNO diffusion from GSNO-containing PL/CS hydrogel demonstrated a sustained NO/GSNO release, in concentrations suitable for biomedical applications. The GSNO-PL/CS hydrogel demonstrated a concentration-dependent toxicity to Vero cells, and antimicrobial activity to Pseudomonas aeruginosa (minimum inhibitory concentration and minimum bactericidal concentration values of 0.5 µg·mL -1 of hydrogel, which corresponds to 1 mmol·L -1 of GSNO). Interestingly, the concentration range in which the NO-releasing hydrogel demonstrated an antibacterial effect was not found to be toxic to the Vero mammalian cell. Thus, the GSNO-PL/CS hydrogel is a suitable biomaterial for topical NO delivery applications. |
